Tested Applications
| Positive WB detected in | HeLa cells, HK-2 cells, L02 cells, mouse lung tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15054-1-AP targets AKR1A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7037 Product name: Recombinant human AKR1A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-325 aa of BC000670 Sequence: MAASCVLLHTGQKMPLIGLGTWKSEPGQVKAAVKYALSVGYRHIDCAAIYGNEPEIGEALKEDVGPGKAVPREELFVTSKLWNTKHHPEDVEPALRKTLADLQLEYLDLYLMHWPYAFERGDNPFPKNADGTICYDSTHYKETWKALEALVAKGLVQALGLSNFNSRQIDDILSVASVRPAVLQVECHPYLAQNELIAHCQARGLEVTAYSPLGSSDRAWRDPDEPVLLEEPVVLALAEKYGRSPAQILLRWQVQRKVICIPKSITPSRILQNIKVFDFTFSPEEMKQLNALNKNWRYIVPMLTVDGKRVPRDAGHPLYPFNDPY Predict reactive species |
| Full Name | aldo-keto reductase family 1, member A1 (aldehyde reductase) |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC000670 |
| Gene Symbol | AKR1A1 |
| Gene ID (NCBI) | 10327 |
| RRID | AB_2224138 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14550 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AKR1A1 antibody 15054-1-AP | Download protocol |
| IHC protocol for AKR1A1 antibody 15054-1-AP | Download protocol |
| IP protocol for AKR1A1 antibody 15054-1-AP | Download protocol |
| WB protocol for AKR1A1 antibody 15054-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-AKR1A1 (SCoR2) antibody, catalog number 15054-1-AP, has 9 total citations published in Nature, Molecular Cell, Journal of Allergy and Clinical Immunology, Journal of Medicinal Chemistry, Science Signaling, FEBS Journal, Journal of Biological Chemistry, eLife, and bioRxiv. Research fields span metabolic reprogramming in kidney and cardiac injury, nitric oxide signaling, asthma pathophysiology, lipogenesis, vitamin B6 metabolism, and S-nitroso-CoA reductase biology.
| Species | Application | Title |
|---|---|---|
Nature Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury.
| ||
J Allergy Clin Immunol Inhaled S-nitrosoglutathione in asthma: Airway AKR1A1 expression and determinants of the human bronchodilator response. | ||
J Med Chem Identification of a Selective SCoR2 Inhibitor That Protects Against Acute Kidney Injury | ||
Sci Signal The protein denitrosylase SCoR2 regulates lipogenesis and fat storage.
| ||
FEBS J A new branch of mammalian vitamin B(6) metabolism: AKR1C-mediated conversion of pyridoxal to pyridoxine and 4-pyridoxolactone. |
