Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, C6 cells, C2C12 cells, mouse brain tissue, rat brain tissue, Jurkat cells, RAW 264, HSC-T6 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10176-2-AP targets AKT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, canine, chicken, bovine, hamster, goat, fish, duck, megalobrama amblycephala |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0213 Product name: Recombinant human AKT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-224 aa of BC000479 Sequence: MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLC Predict reactive species |
| Full Name | v-akt murine thymoma viral oncogene homolog 1 |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 56-62 kDa |
| GenBank Accession Number | BC000479 |
| Gene Symbol | AKT1 |
| Gene ID (NCBI) | 207 |
| RRID | AB_2224574 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P31749 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AKT antibody 10176-2-AP | Download protocol |
| IHC protocol for AKT antibody 10176-2-AP | Download protocol |
| IP protocol for AKT antibody 10176-2-AP | Download protocol |
| WB protocol for AKT antibody 10176-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-AKT (Cat# 10176-2-AP) has accumulated 2,311 citations across high-impact journals including Nature Communications, Cell Research, Cancer Research, Cell Death & Disease, Advanced Science, Molecular Cancer, Phytomedicine, and J Nanobiotechnology. Research fields span oncology (colorectal, breast, glioblastoma, lung, gastric, pancreatic, and ovarian cancers), PI3K/AKT/mTOR signaling, metabolism, neurology, cardiology, immunology, autophagy, ferroptosis, tissue regeneration, diabetes, and nanomedicine. The antibody is predominantly applied in Western blot and IHC.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Overexpression of STX11 alleviates pulmonary fibrosis by inhibiting fibroblast activation via the PI3K/AKT/mTOR pathway | ||
J Hematol Oncol A novel lncRNA ROPM-mediated lipid metabolism governs breast cancer stem cell properties. | ||
J Hematol Oncol Long non-coding RNA-SNHG7 acts as a target of miR-34a to increase GALNT7 level and regulate PI3K/Akt/mTOR pathway in colorectal cancer progression. | ||
J Hematol Oncol A cytoplasmic long noncoding RNA LINC00470 as a new AKT activator to mediate glioblastoma cell autophagy. | ||
Mol Cancer EIF4A3/circPTGR1/miR-4725-5p positive- feedback loop promotes colorectal cancer progression via FAK/AKT signaling pathway. | ||
Mol Cancer Circular RNA circSLC8A1 acts as a sponge of miR-130b/miR-494 in suppressing bladder cancer progression via regulating PTEN. |
Reviews
What customers say
All three reviews are for Western Blot applications and are positive. Users report good staining results across different cell lines (pancreatic cancer, HAP1, NSC34). Recommended dilutions range from 1:500 to 1:2000. One user suggested using a slightly higher dilution than 1:2000 to reduce background noise. Overall, the antibody is well-received for WB with consistent performance across multiple cell types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ana (Verified Customer) (06-17-2025) | The staining looks very good. It might be better to dilute a bit more than 1:2000 to reduce background noise.
|
Hyopil (Verified Customer) (08-23-2021) | Worked well with western blotting
|
Azita (Verified Customer) (06-02-2021) | Western blot analysis using AKT polyclonal antibody in NSC34 cell line at dilution of 1:500.
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