AKT Antibody
Rabbit Polyclonal
Catalog number: 10176-2-AP
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Applications
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | WB |
| Species specificity | human, mouse, rat, zebrafish; other species not tested. |
| Cited species | human |
| Positive WB detected in | HeLa cells, A549 cells, HepG2 cells, human liver tissue, mouse testis tissue |
| Positive IHC detected in | human cervical cancer |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of anti-AKT1 (10176-2-AP for IP and Detection) with HeLa cell lysate.
siRNA AKT1 result from Dr. Eva Martinez-Balibrea. Green:tubulin, Red:10176-2-AP, AKT1.
Control:siRNA AKT1 result with Pan-AKT antibody.From Dr.Eva Martinez-Balibrea.
HeLa cells were subjected to SDS PAGE followed by western blot with 10176-2-AP(AKT Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human cervical cancer using 10176-2-AP(AKT Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human cervical cancer using 10176-2-AP(AKT Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | AKT fusion protein ag0213 | Full name | v-akt murine thymoma viral oncogene homolog 1 |
|
Calculated molecular weight |
62kd |
Observed molecular
weight |
62kd |
|
GenBank accession number |
BC000479 | Gene ID (NCBI) | 207 |
| Gene symbol | AKT1 | Synonyms | AKT; MGC99656; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA |
Background
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. |
