AKT Antibody
Mouse Monoclonal
Catalog number: 60203-2-Ig
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human, mouse; other species not tested. |
| Cited species | |
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig(AKT Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human cervical cancer using 60203-2-Ig(AKT Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human cervical cancer using 60203-2-Ig(AKT Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Mouse | Purification method | Protein A purification |
| Isotype | IgG1 | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | AKT fusion protein ag16695 | Full name | v-akt murine thymoma viral oncogene homolog 1 |
|
Calculated molecular weight |
62kd |
Observed molecular
weight |
62kd |
|
GenBank accession number |
BC000479 | Gene ID (NCBI) | 207 |
| Gene symbol | AKT1 | Synonyms | AKT; MGC99656; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA |
Background
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. |
