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AKT Polyclonal antibody

AKT Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 51077-1-AP

Synonyms

AKT, AKT1, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha



Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, A549 cells, COLO 320 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51077-1-AP targets AKT in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56-62 kDa
GenBank Accession NumberBC084538
Gene Symbol AKT1
Gene ID (NCBI) 207
RRIDAB_2242475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Protocols

Product Specific Protocols
WB protocol for AKT antibody 51077-1-APDownload protocol
IHC protocol for AKT antibody 51077-1-APDownload protocol
IF protocol for AKT antibody 51077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

Binding blockade between TLN1 and integrin β1 represses triple-negative breast cancer.

Authors - Yixiao Zhang
humanWB

Front Oncol

Low Expression Levels of SLC22A12 Indicates a Poor Prognosis and Progresses Clear Cell Renal Cell Carcinoma.

Authors - Jiaju Xu
humanWB

Biomed Pharmacother

α-Terthienyl induces prostate cancer cell death through inhibiting androgen receptor expression.

Authors - Xia Gan
mouseWB

Sci Rep

Aronia melanocarpa polysaccharide ameliorates inflammation and aging in mice by modulating the AMPK/SIRT1/NF-κB signaling pathway and gut microbiota

Authors - Yingchun Zhao
ratWB

Front Cell Neurosci

Healthy Serum-Derived Exosomes Improve Neurological Outcomes and Protect Blood-Brain Barrier by Inhibiting Endothelial Cell Apoptosis and Reversing Autophagy-Mediated Tight Junction Protein Reduction in Rat Stroke Model.

Authors - Lin-Yan Huang
humanWB

J Cell Biochem

Combined detection of estrogen and tumor markers is an important reference factor in the diagnosis and prognosis of lung cancer.

Authors - Yuquan Bai