Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14860-1-AP targets AKTIP in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6662 Product name: Recombinant human AKTIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-292 aa of BC001134 Sequence: MNPFWSMSTSSVRKRSEGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPAAQSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIEDPYAISFSPWNPSVHDEAREKMLTQKKPEEQHNKSVHVAGLSWVKPGSVQPFSKEEKTVAT Predict reactive species |
| Full Name | AKT interacting protein |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC001134 |
| Gene Symbol | AKTIP |
| Gene ID (NCBI) | 64400 |
| RRID | AB_2878087 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H8T0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AKTIP is a telomeric protein with the property of being enriched at the nuclear lamina. AKTIP has similarity with the tumor susceptibility gene TSG101. AKTIP deficiency generates genome instability and, in p53−/− mice, the reduction of the mouse counterpart of AKTIP induces the exacerbation of lymphomas.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AKTIP antibody 14860-1-AP | Download protocol |
| IP protocol for AKTIP antibody 14860-1-AP | Download protocol |
| WB protocol for AKTIP antibody 14860-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AKTIP polyclonal antibody (Cat# 14860-1-AP) has 2 citations. These appear in Cell Reports and Scientific Reports. The cited studies focus on breast cancer (specifically ERα-positive breast cancer tumorigenesis and endocrine resistance) and lung adenocarcinoma (SYT15 expression and molecular mechanisms), both utilizing Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Cell Rep AKTIP loss is enriched in ERα-positive breast cancer for tumorigenesis and confers endocrine resistance
| ||
Sci Rep Clinical significance and potential molecular mechanisms of SYT15 expression in lung adenocarcinoma. |
