Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, Raji cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16200-1-AP targets ALAS1 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9126 Product name: Recombinant human ALAS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-330 aa of BC011798 Sequence: MESVVRRCPFLSRVPQAFLQKAGKSLLFYAQNCPKMMEVGAKPAPRALSTAAVHYQQIKETPPASEKDKTAKAKVQQTPDGSQQSPDGTQLPSGHPLPATSQGTASKCPFLAAQMNQRGSSVFCKASLELQEDVQEMNAVRKEVAETSAGPSVVSVKTDGGDPSGLLKNFQDIMQKQRPERVSHLLQDNLPKSVSTFQYDRFFEKKIDEKKNDHTYRVFKTVNRRAHIFPMADDYSDSLITKKQVSVWCSNDYLGMSRHPRVCGAVMDTLKQHGAGAGGTRNISGTSKFHVDLERELADLHGKDAALLFSSCFVANDSTLFTLAKMMPGC Predict reactive species |
| Full Name | aminolevulinate, delta-, synthase 1 |
| Calculated Molecular Weight | 640 aa, 71 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC011798 |
| Gene Symbol | ALAS1 |
| Gene ID (NCBI) | 211 |
| RRID | AB_2878227 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13196 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ALAS1 antibody 16200-1-AP | Download protocol |
| WB protocol for ALAS1 antibody 16200-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-ALAS1 antibody (Cat# 16200-1-AP) has 13 total citations. These appear in journals including Nature, Cell, Molecular Cell, Redox Biology, Genome Medicine, Nature Metabolism, Free Radical Biology and Medicine, Journal of Neurosurgery, and others. Research fields span mitochondrial biology, heme biosynthesis, ferroptosis, oxidative stress, cancer biology, cardiovascular disease, muscle physiology, hepatotoxicity, and reproductive biology. Applications include Western blotting and immunofluorescence across human, mouse, rat, and pig species.
| Species | Application | Title |
|---|---|---|
Redox Biol Txnrd2 loss in skeletal muscle causes muscle atrophy and drives leanness and obesity resistance. | ||
Mol Cell Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability. | ||
Free Radic Biol Med Heme-mediated liver-heart axis promotes myocardial pyroptosis in db/db mice. |
