Tested Applications
| Positive WB detected in | HEK-293T cells, HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
Product Information
15090-1-AP targets ALDH3A2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1687 Product name: Recombinant human ALDH3A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-314 aa of BC002430 Sequence: MELEVRRVRQAFLSGRSRPLRFRLQQLEALRRMVQEREKDILTAIAADLCKSEFNVYSQEVITVLGEIDFMLENLPEWVTAKPVKKNVLTMLDEAYIQPQPLGVVLIIGAWNYPFVLTIQPLIGAIAAGNAVIIKPSELSENTAKILAKLLPQYLDQDLYIVINGGVEETTELLKQRFDHIFYTGNTAVGKIVMEAAAKHLTPVTLELGGKSPCYIDKDCDLDIVCRRITWGKYMNCGQTCIAPDYILCEASLQNQIVWKIKETVKEFYGENIKESPDYERIINLRHFKRILSLLEGQKIAFGGETDEATRYIA Predict reactive species |
| Full Name | aldehyde dehydrogenase 3 family, member A2 |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 50-57 kDa |
| GenBank Accession Number | BC002430 |
| Gene Symbol | ALDH3A2 |
| Gene ID (NCBI) | 224 |
| RRID | AB_2224316 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51648 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ALDH3A2 antibody 15090-1-AP | Download protocol |
| IF protocol for ALDH3A2 antibody 15090-1-AP | Download protocol |
| IHC protocol for ALDH3A2 antibody 15090-1-AP | Download protocol |
| IP protocol for ALDH3A2 antibody 15090-1-AP | Download protocol |
| WB protocol for ALDH3A2 antibody 15090-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Transl Med Deficiency of SDHC promotes metastasis by reprogramming fatty acid metabolism in colorectal cancer | ||
J Cell Mol Med Taraxacum officinale extract ameliorates dextran sodium sulphate-induced colitis by regulating fatty acid degradation and microbial dysbiosis. | ||
Cells Aldehyde Dehydrogenase, a Therapeutic Target in Chordoma: Analysis in 3D Cellular Models. | ||
J Biol Chem Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways. | ||
Int J Mol Sci Targeting Retinaldehyde Dehydrogenases to Enhance Temozolomide Therapy in Glioblastoma | ||
Front Med (Lausanne) Synthetic antibody-derived immunopeptide provides neuroprotection in glaucoma through molecular interaction with retinal protein histone H3.1 |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Elisabeth (Verified Customer) (09-02-2019) | HeLa cells treated with control (Lane 1) or ALDH3A2 (Lane 2) siRNA for 72h.
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