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  • Featured Product
  • KD/KO Validated

ALDH5A1 Polyclonal antibody

ALDH5A1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

Cat no : 17319-1-AP

Synonyms

ALDH5A1, SSADH, SSDH

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALDH5A1 Polyclonal antibody (17319-1-AP)

    WB analysis using 17319-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17319-1-AP (ALDH5A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ALDH5A1 Polyclonal antibody (17319-1-AP)

    WB analysis of HepG2 using 17319-1-AP

    WB result of ALDH5A1 antibody (17319-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH5A1 transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ALDH5A1 Polyclonal antibody (17319-1-AP)

    IHC staining of human liver cancer using 17319-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ALDH5A1 Polyclonal antibody (17319-1-AP)

    IHC staining of human liver cancer using 17319-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ALDH5A1 Polyclonal antibody (17319-1-AP)

    IHC staining of human liver cancer using 17319-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ALDH5A1 Polyclonal antibody (17319-1-AP)

    IHC staining of human liver cancer using 17319-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ALDH5A1 Polyclonal antibody (17319-1-AP)

    IHC staining of human liver using 17319-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17319-1-AP (ALDH5A1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ALDH5A1 Polyclonal antibody (17319-1-AP)

    IHC staining of human liver using 17319-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17319-1-AP (ALDH5A1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ALDH5A1 Polyclonal antibody (17319-1-AP)

    IF Staining of A431 using 17319-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17319-1-AP targets ALDH5A1 in WB,IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH5A1 fusion protein Ag11293
Full Name aldehyde dehydrogenase 5 family, member A1
Calculated Molecular Weight 535 aa, 57 kDa
Observed Molecular Weight 51-59 kDa
GenBank Accession NumberBC034321
Gene Symbol ALDH5A1
Gene ID (NCBI) 7915
RRIDAB_2224478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ALDH5A1 antibody 17319-1-APDownload protocol
IHC protocol for ALDH5A1 antibody 17319-1-APDownload protocol
IF protocol for ALDH5A1 antibody 17319-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Biol Ther

Decreased expression of ALDH5A1 predicts prognosis in patients with ovarian cancer.

Authors - Xun Tian
View Article
mouseWB

Neuropharmacology

A role of GABAA receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine

Authors - Xiao-Hui Tang
View Article
human,mouseWB,IF

Aging (Albany NY)

Mitochondrial-related genes PDK2, CHDH, and ALDH5A1 served as a diagnostic signature and correlated with immune cell infiltration in ulcerative colitis

Authors - Qian Yang
View Article