ALDOB Antibody
Rabbit Polyclonal
Catalog number: 18065-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human liver |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP(ALDOB Antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human liver using 18065-1-AP(ALDOB Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human liver using 18065-1-AP(ALDOB Antibody) at Dilution 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using ALDOB antibody 18065-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | ALDOB fusion protein ag12695 | Full name | aldolase B, fructose-bisphosphate |
|
Calculated molecular weight |
316aa,35kd |
Observed molecular
weight |
40kd |
|
GenBank accession number |
BC029399 | Gene ID (NCBI) | 229 |
| Gene symbol | ALDOB | Synonyms |
Background
Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A , B(ALDOB), and C. Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody is specific to ALDOB. |
