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  • Featured Product
  • KD/KO Validated

ALKBH5 Polyclonal antibody

ALKBH5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, RIP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16837-1-AP

Synonyms

ABH5, ALKBH5, OFOXD, OFOXD1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ALKBH5 Polyclonal antibody (16837-1-AP)

    WB analysis using 16837-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16837-1-AP (ALKBH5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ALKBH5 Polyclonal antibody (16837-1-AP)

    WB analysis of HeLa using 16837-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16837-1-AP (ALKBH5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ALKBH5 Polyclonal antibody (16837-1-AP)

    WB analysis of HEK-293 using 16837-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16837-1-AP (ALKBH5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ALKBH5 Polyclonal antibody (16837-1-AP)

    IHC staining of human testis using 16837-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ALKBH5 Polyclonal antibody (16837-1-AP)

    IHC staining of human testis using 16837-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ALKBH5 Polyclonal antibody (16837-1-AP)

    IHC staining of human lung cancer using 16837-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ALKBH5 Polyclonal antibody (16837-1-AP)

    IHC staining of human lung cancer using 16837-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16837-1-AP targets ALKBH5 in WB, RIP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALKBH5 fusion protein Ag10516
Full Name alkB, alkylation repair homolog 5 (E. coli)
Calculated Molecular Weight 458 aa, 51 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC062339
Gene Symbol ALKBH5
Gene ID (NCBI) 54890
RRIDAB_2242665
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.

Protocols

Product Specific Protocols
WB protocol for ALKBH5 antibody 16837-1-APDownload protocol
IHC protocol for ALKBH5 antibody 16837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Genet

N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.

Authors - Yuan Li
View Article
humanIF

Cell Res

The m6A methylome of SARS-CoV-2 in host cells.

Authors - Jun'e Liu
  • KD Validated
View Article
mouseWB,RIP

Nat Cell Biol

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.

Authors - Xiang-Qian Gao
View Article
humanWB

Nat Commun

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis

Authors - Jian Zhong
View Article
humanWB

Mol Cell

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Authors - Elodie Villa
View Article
humanRIP

Mol Cancer

Demethylase ALKBH5 suppresses invasion of gastric cancer via PKMYT1 m6A modification.

Authors - Yiyang Hu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

PK (Verified Customer) (07-08-2022)

GOOD

  • Applications: Western Blot
  • Cell Tissue Type: H9C2
ALKBH5 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:16837-1-AP)
FH

SU (Verified Customer) (03-22-2022)

  • Applications: Western Blot
  • Cell Tissue Type: H9C2
ALKBH5 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:16837-1-AP)
FH

ANIRBAN (Verified Customer) (04-07-2021)

Tried to validate the antibody (AlkBH5) in two different cell lines (786-o, HK2) using three siRNA constructs from IDT. Using the Proteintech antibody, I didn't see any evedence of KD in the lysates (see the figure attached). When I used the Cell Signaling antibody for AlkBH5 (#80283) I observed a clear evedence of KD in the same lysates that I mentioned before. scr=scrambled; si-1,2,3=siRNAs targeting AlkBH5.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2K
  • Cell Tissue Type: RCC cell lines
ALKBH5 Antibody Western Blot validation (1:2K dilution) in RCC cell lines (Cat no:16837-1-AP)
FH

Bill (Verified Customer) (12-23-2019)

This antibody has some unspecific band, but is ok to detect ALKBH5, siRNA validated.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: colerectal cancer cells
FH

Daniel (Verified Customer) (12-04-2019)

Staining of kidney proximal tubules

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse kidney (TRACK model)
ALKBH5 Antibody Immunohistochemistry, validation (1:200 dilution) in Mouse kidney (TRACK model) (Cat no:16837-1-AP)