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WB analysis using 16837-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16837-1-AP (ALKBH5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 16837-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16837-1-AP (ALKBH5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16837-1-AP
IP result of anti-ALKBH5 (IP:16837-1-AP, 4ug; Detection:16837-1-AP 1:8000) with HeLa cells lysate 1280 ug.
IHC staining of human testis using 16837-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 16837-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of mouse testis using 16837-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16837-1-AP (ALKBH5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse testis using 16837-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using ALKBH5 antibody (16837-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

ALKBH5 Polyclonal antibody

Name: ALKBH5 Polyclonal antibody
Brand: Proteintech
SKU: 16837-1-AP
Rating: 4.00 (5 reviews)

ALKBH5 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 16837-1-AP

Publications(204)

Reviews (5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF-P, IP, CoIP, RIP, ELISA

Synonyms

ALKBH 5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, EC:1.14.11.53, RNA demethylase ALKBH5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information
Publications (204)
Protocols
Reviews (5)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, HeLa cells, Calu-1 cells, mouse brain tissue, rat brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human testis tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 20 publications below
WB	See 180 publications below
IHC	See 29 publications below
IF	See 16 publications below
IP	See 1 publications below
CoIP	See 2 publications below
RIP	See 17 publications below

Product Information

16837-1-AP targets ALKBH5 in WB, IHC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10516 Product name: Recombinant human ALKBH5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 57-383 aa of BC062339 Sequence: KRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCSEAAG Predict reactive species
Full Name	alkB, alkylation repair homolog 5 (E. coli)
Calculated Molecular Weight	458 aa, 51 kDa
Observed Molecular Weight	40-50 kDa
GenBank Accession Number	BC062339
Gene Symbol	ALKBH5
Gene ID (NCBI)	54890
RRID	AB_2242665
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6P6C2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.

Protocols

Product Specific Protocols
IHC protocol for ALKBH5 antibody 16837-1-AP	Download protocol
IP protocol for ALKBH5 antibody 16837-1-AP	Download protocol
WB protocol for ALKBH5 antibody 16837-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Genet N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2. Authors - Yuan Li View Article
human	IF	Cell Res The m6A methylome of SARS-CoV-2 in host cells. Authors - Jun'e Liu KD Validated View Article
mouse	WB,RIP	Nat Cell Biol The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA. Authors - Xiang-Qian Gao View Article
human	WB	Nat Commun Circular RNA encoded MET variant promotes glioblastoma tumorigenesis Authors - Jian Zhong View Article
mouse	WB	Nat Commun ALKBH5-mediated m6A modification of IL-11 drives macrophage-to-myofibroblast transition and pathological cardiac fibrosis in mice Authors - Tao Zhuang View Article
mouse,human	WB	Nat Commun Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma Authors - Weiwei Dai View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH PK (Verified Customer) (07-08-2022)	GOOD Applications: Western Blot Cell Tissue Type: H9C2 ALKBH5 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:16837-1-AP)
FH SU (Verified Customer) (03-22-2022)	Applications: Western Blot Cell Tissue Type: H9C2 ALKBH5 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:16837-1-AP)
FH ANIRBAN (Verified Customer) (04-07-2021)	Tried to validate the antibody (AlkBH5) in two different cell lines (786-o, HK2) using three siRNA constructs from IDT. Using the Proteintech antibody, I didn't see any evedence of KD in the lysates (see the figure attached). When I used the Cell Signaling antibody for AlkBH5 (#80283) I observed a clear evedence of KD in the same lysates that I mentioned before. scr=scrambled; si-1,2,3=siRNAs targeting AlkBH5. Applications: Western Blot Primary Antibody Dilution: 1:2K Cell Tissue Type: RCC cell lines ALKBH5 Antibody Western Blot validation (1:2K dilution) in RCC cell lines (Cat no:16837-1-AP)
FH Bill (Verified Customer) (12-23-2019)	This antibody has some unspecific band, but is ok to detect ALKBH5, siRNA validated. Applications: Western Blot, Primary Antibody Dilution: 1:2000 Cell Tissue Type: colerectal cancer cells
FH Daniel (Verified Customer) (12-04-2019)	Staining of kidney proximal tubules Applications: Immunohistochemistry, Primary Antibody Dilution: 1:200 Cell Tissue Type: Mouse kidney (TRACK model) ALKBH5 Antibody Immunohistochemistry, validation (1:200 dilution) in Mouse kidney (TRACK model) (Cat no:16837-1-AP)