ALOX5 Antibody
Rabbit Polyclonal
Catalog number: 10021-1-Ig
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Applications
| Tested applications | ELISA, IF |
| Cited applications | |
| Species specificity | human, mouse; other species not tested. |
| Cited species | |
| Positive IF detected in | Hela cells |
| Recommended dilution | IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Immunofluorescent analysis of Hela cells, using ALOX5 antibody 10021-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Protein A purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Recombinant Protein | Full name | arachidonate 5-lipoxygenase |
|
Calculated molecular weight |
674aa,78kd |
Observed molecular
weight |
78kd |
|
GenBank accession number |
BC130332 | Gene ID (NCBI) | 240 |
| Gene symbol | ALOX5 | Synonyms | 5-LO; 5-LOX; 5LPG; LOG5; MGC163204 |
Background
ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785). This antibody is specific to ALOX5. |
