ALS2 Polyclonal antibody

ALS2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13998-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

ALS2CR6, Alsin, ALSJ, IAHSP, PLSJ

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis using 13998-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13998-1-AP (ALS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 13998-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13998-1-AP (ALS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HEK-293 using 13998-1-AP
IP result of anti-ALS2 (IP:13998-1-AP, 4ug; Detection:13998-1-AP 1:800) with HEK-293 cells lysate 1920 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 13998-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13998-1-AP (ALS2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 13998-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13998-1-AP (ALS2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 13998-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13998-1-AP (ALS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human rectal cancer using 13998-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 13998-1-AP (ALS2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 13998-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13998-1-AP (ALS2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse cerebellum tissue, HEK-293 cells, rat cerebellum tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	mouse cerebellum tissue, rat kidney tissue, human rectal cancer tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13998-1-AP targets ALS2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5087 Product name: Recombinant human ALS2 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-304 aa of BC029174 Sequence: MDSKKRSSTEAEGSKERGLVHIWQAGSFPITPERLPGWGGKTVLQAALGVKHGVLLTEDGEVYSFGTLPWRSGPVEICPSSPILENALVGQYVITVATGSFHSGAVTDNGVAYMCGENSAGQCAVANQQYVPEPNPVSIADSEASPLLAVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTAFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLTESQAENHASTALSPSTETLDRQEEVFENTLVANDQSVATE Predict reactive species
Full Name	amyotrophic lateral sclerosis 2 (juvenile)
Calculated Molecular Weight	1657 aa, 184 kDa
Observed Molecular Weight	185 kDa
GenBank Accession Number	BC029174
Gene Symbol	ALS2
Gene ID (NCBI)	57679
RRID	AB_10858476
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96Q42
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for ALS2 antibody 13998-1-AP	Download protocol
IP protocol for ALS2 antibody 13998-1-AP	Download protocol
WB protocol for ALS2 antibody 13998-1-AP	Download protocol

Standard Protocols
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Publications

What published studies show

The ALS2 polyclonal antibody (Cat# 13998-1-AP) has a total of 2 citations. Both appear in Neurobiology of Disease and bioRxiv, published by Brianna Hnath et al. The research focuses on a novel extracellular vesicle release pathway facilitated by toxic superoxide dismutase 1 (SOD1) oligomers, placing the work within the neurodegeneration and extracellular vesicle biology fields. Applications cited include Western blotting and ELISA in human samples.

Species	Application	Title
human	WB,ELISA	Neurobiol Dis Novel extracellular vesicle release pathway facilitated by toxic superoxide dismutase 1 oligomers. Authors - Brianna Hnath View Article
	WB	bioRxiv Novel extracellular vesicle release pathway facilitated by toxic superoxide dismutase 1 oligomers Authors - Brianna Hnath View Article