ALX1 Polyclonal antibody

ALX1 Polyclonal Antibody for WB, ELISA

Cat No. 16372-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

ALX homeobox 1, ALX homeobox protein 1, ALX1, CART 1, CART1, Cartilage homeoprotein 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWB

WB analysis of mouse embryo using 16372-1-AP
mouse embryo tissue were subjected to SDS PAGE followed by western blot with 16372-1-AP (ALX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of ROS1728 using 16372-1-AP
ROS1728 cells were subjected to SDS PAGE followed by western blot with 16372-1-AP (ALX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

mouse embryo tissue, ROS1728 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

16372-1-AP targets ALX1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9295 Product name: Recombinant human ALX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-326 aa of BC010923 Sequence: MEFLSEKFALKSPPSKNSDFYMGAGGPLEHVMETLDNESFYSKASAGKCVQAFGPLPRAEHHVRLERTSPCQDSSVNYGITKVEGQPLHTELNRAMDNCNSLRMSPVKGMQEKGELDELGDKCDSNVSSSKKRRHRTTFTSLQLEELEKVFQKTHYPDVYVREQLALRTELTEARVQVWFQNRRAKWRKRERYGQIQQAKSHFAATYDISVLPRTDSYPQIQNNLWAGNASGGSVVTSCMLPRDTSSCMTPYSHSPRTDSSYTGFSNHQNQFSHVPLNNFFTDSLLTGATNGHAFETKPEFERRSSSIAVLRMKAKEHTANISWAM Predict reactive species
Full Name	ALX homeobox 1
Calculated Molecular Weight	326 aa, 37 kDa
Observed Molecular Weight	35-37 kDa
GenBank Accession Number	BC010923
Gene Symbol	ALX1
Gene ID (NCBI)	8092
RRID	AB_2878249
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15699
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
WB protocol for ALX1 antibody 16372-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
mouse	WB,IF	Front Cell Dev Biol Alx1 Deficient Mice Recapitulate Craniofacial Phenotype and Reveal Developmental Basis of ALX1-Related Frontonasal Dysplasia. Authors - Paul P R Iyyanar KO Validated View Article
mouse	IF	Dis Model Mech The ALX1 transcription factor acts in the early cranial mesoderm to specify extraocular muscle formation. Authors - Paul P. R. Iyyanar View Article