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  • Featured Product
  • KD/KO Validated

ALX1 Polyclonal antibody

ALX1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16372-1-AP

Synonyms

ALX homeobox 1, ALX homeobox protein 1, ALX1, CART 1, CART1, Cartilage homeoprotein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse embryo tissue using ALX1 Polyclonal antibody (16372-1-AP)

    WB analysis of mouse embryo using 16372-1-AP

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 16372-1-AP (ALX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using ALX1 Polyclonal antibody (16372-1-AP)

    WB analysis of ROS1728 using 16372-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 16372-1-AP (ALX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse embryo tissue, ROS1728 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 16372-1-AP is ALX1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALX1 fusion protein Ag9295
Full Name ALX homeobox 1
Calculated Molecular Weight 326 aa, 37 kDa
Observed Molecular Weight 35-37 kDa
GenBank Accession NumberBC010923
Gene Symbol ALX1
Gene ID (NCBI) 8092
RRIDAB_2878249
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ALX1 antibody 16372-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Front Cell Dev Biol

Alx1 Deficient Mice Recapitulate Craniofacial Phenotype and Reveal Developmental Basis of ALX1-Related Frontonasal Dysplasia.

Authors - Paul P R Iyyanar
  • KO Validated
View Article