Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, A431 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16690-1-AP targets ALY in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10044 Product name: Recombinant human ALY protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 5-257 aa of BC052302 Sequence: MDMSLDDIIKLNRSQRGGRGGGRGRGRAGSQGGRGGGAQAAARVNRGGGPIRNRPAIARGAAGGGGRNRPAPYSRPKQLPDKWQHDLFDSGFGGGAGVETGGKLLVSNLDFGVSDADIQELFAEFGTLKKAAVHYDRSGRSLGTADVHFERKADALKAMKQYNGVPLDGRPMNIQLVTSQIDAQRRPAQSVNRGGMTRNRGAGGFGGGGGTRRGTRGGARGRGRGAGRNSKQQLSAEELDAQLDAYNARMDTS Predict reactive species |
| Full Name | THO complex 4 |
| Calculated Molecular Weight | 257 aa, 27 kDa |
| Observed Molecular Weight | 27-30 kDa |
| GenBank Accession Number | BC052302 |
| Gene Symbol | ALY |
| Gene ID (NCBI) | 10189 |
| RRID | AB_2878300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86V81 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ALY antibody 16690-1-AP | Download protocol |
| IHC protocol for ALY antibody 16690-1-AP | Download protocol |
| WB protocol for ALY antibody 16690-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Cell Metab NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. | ||
ACS Infect Dis Porcine ZC3H11A Is Essential for the Proliferation of Pseudorabies Virus and Porcine Circovirus 2.
| ||
NPJ Precis Oncol FOXA1-dependent NSUN2 facilitates the advancement of prostate cancer by preserving TRIM28 mRNA stability in a m5C-dependent manner | ||
Sci Rep Comprehensive analysis of ALYREF gene expression and its correlation with immunotherapy efficacy and circulating tumor cells in bladder cancer. | ||
Exp Cell Res ALYREF drives NLRP3 inflammasome-dependent macrophage pyroptosis to promote sepsis-associated acute liver injury by enhancing GADD45A mRNA stability. | ||
PLoS Pathog TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus
|
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Roy (Verified Customer) (06-15-2024) | Weak band on WB from HeLa cells extracts but specific for ALYREF/THOC4 (1/1000 dilution, Overnight incubation at 4°C)
|
