AMH Polyclonal antibody

AMH Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14461-1-AP

Synonyms

AMH, anti Mullerian hormone, MIF, MIS



Tested Applications

Positive WB detected inhuman testis tissue
Positive IHC detected inmouse ovary tissue, human pancreas tissue, rat ovary tissue, human ovary tumor tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse ovary tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14461-1-AP targets AMH in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat, goat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMH fusion protein Ag5670
Full Name anti-Mullerian hormone
Calculated Molecular Weight 59 kDa
GenBank Accession NumberBC049194
Gene Symbol AMH
Gene ID (NCBI) 268
RRIDAB_10915986
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance (MIS), is a hormone that is best known for its production by fetal testes in mammals and as the inhibitor of Müllerian duct development in males. AMH is also expressed in granulosa cells of preantral and small antral follicles in the ovary. More recently, AMH has been studied for its role in ovarian folliculogenesis and as a potential marker of ovarian reserve. The deduced protein sequence of the AMH monomer contains a 25 amino acid secretion specific signal peptide and a monomeric protein of 535 amino acids that, when glycosylated at two glycosylation sites, has a molecular weight of 65-70 kDa. This antibody is specific to AMH.

Protocols

Product Specific Protocols
WB protocol for AMH antibody 14461-1-APDownload protocol
IHC protocol for AMH antibody 14461-1-APDownload protocol
IF protocol for AMH antibody 14461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chickenWB

Theriogenology

miR-140-3p promotes follicle granulosa cell proliferation and steroid hormone synthesis via targeting AMH in chickens

Authors - Bei-Bei Zhang
  • KD Validated
mouseWB

Exp Cell Res

Proteomic analysis of mouse ovaries during the prepubertal stages.

Authors - Jiaqiang Xiong

Hum Reprod

Peroxisome proliferator-activated receptor-γ coactivator 1α-mediated pathway as a possible therapeutic target in endometriosis.

Authors - Hisashi Kataoka
mouseIHC

J Funct Biomater

Construction of Artificial Ovaries with Decellularized Porcine Scaffold and Its Elicited Immune Response after Xenotransplantation in Mice

Authors - Tong Wu
ratWB

Basic Clin Pharmacol Toxicol

Effects of quercetin on ovarian function and regulation of the ovarian PI3K/Akt/FoxO3a signalling pathway and oxidative stress in a rat model of cyclophosphamide-induced premature ovarian failure

Authors - Shaoyan Zheng
goatWB

Theriogenology

BMP6 regulates AMH expression via SMAD1/5/8 in goat ovarian granulosa cells

Authors - Lei Wang