Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19780-1-AP targets AMPD1-Specific in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | adenosine monophosphate deaminase 1 (isoform M) |
| Calculated Molecular Weight | 87 kDa |
| Observed Molecular Weight | 75-83 kDa |
| GenBank Accession Number | NM_000036 |
| Gene Symbol | AMPD1 |
| Gene ID (NCBI) | 270 |
| RRID | AB_10644281 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23109 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AMPD1, also named as MAD and MADA, belongs to the adenosine and AMP deaminases family. AMP deaminase plays a critical role in energy metabolism. It catalyzes the reaction: AMP + H2O = IMP + NH3. Defects in AMPD1 are the cause of adenosine monophosphate deaminase deficiency muscle type (AMPDDM) which is a metabolic disorder resulting in exercise-related myopathy. The antibody is specific to AMPD1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for AMPD1-Specific antibody 19780-1-AP | Download protocol |
| IP protocol for AMPD1-Specific antibody 19780-1-AP | Download protocol |
| WB protocol for AMPD1-Specific antibody 19780-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody targeting AMPD1 (Cat# 19780-1-AP) has 7 total citations across journals including Nature Communications, Science Advances, iScience, J Cell Mol Med, Frontiers in Oncology, Biochem Biophys Rep, and Poult Sci. Research fields span skeletal muscle metabolism, mitochondrial myopathy, cancer immunotherapy, breast cancer prognosis, pancreatic cancer immune infiltration, chronic kidney disease-related sarcopenia, and avian adenylate kinase biology. Applications include WB and IHC in mouse, human, and chicken samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Enhancer regulator MLL4 controls skeletal muscle metabolic efficiency by limiting AMPK-mediated fuel catabolism. | ||
J Cell Mol Med EDNRA regulates the tumour immune environment and predicts the efficacy and prognosis of cancer immunotherapy | ||
iScience Phosphate depletion in insulin-insensitive skeletal muscle drives AMPD activation and sarcopenia in chronic kidney disease
| ||
Poult Sci Investigation of the effects and underlying mechanisms of adenylate kinase 1 on inosine monophosphate deposition in Beijing-You chickens. | ||
Front Oncol AMPD1 Is Associated With the Immune Response and Serves as a Prognostic Marker in HER2-Positive Breast Cancer. |
Reviews
What customers say
One reviewer (Laila K.) gave this antibody a 5-star rating after using it at a 1:1000 dilution for Western Blot on bovine skeletal muscle tissue. She reported excellent performance with only 60 µg of total protein and very little non-specific binding, confirming strong specificity and sensitivity in her application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Laila (Verified Customer) (07-22-2022) | This antibody worked great with Bovine tissue with a total of 60 ug of protein and very little non-specific binding.
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