Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15065-1-AP targets ANAPC11 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7069 Product name: Recombinant human ANAPC11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-196 aa of BC000607 Sequence: MKVKIKCWNGVATWLWVANDENCGICRMAFNGCCPDCPLHGESISRCLGWCPQPVPVLGGRAHPQVPINTASPTPGQHTGSLMSREESSRSPDPTPPALDQETSSLLRCTSPWCLDHSCDLFGITDQVSADGPRACRQGARRRLPAGVGPVLPLLPHALHPQVAARTAGAAALPHVPPGMEVQGVRPDLALAGGAS Predict reactive species |
| Full Name | anaphase promoting complex subunit 11 |
| Calculated Molecular Weight | 21 kDa, 10 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC000607 |
| Gene Symbol | APC11 |
| Gene ID (NCBI) | 51529 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NYG5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Anaphase-promoting complex subunit 11 (ANAPC11) is a core catalytic subunit of the anaphase-promoting complex/cyclosome (APC/C). ANAPC11 is involved in the ubiquitination and degradation of key cell cycle regulators, including cyclin B and securin, facilitating the transition from metaphase to anaphase during cell division (PMID: 11739784; 18485873). ANAPC11 also mediates the ubiquitination and degradation of the Forkhead transcription factor FOXO3, promoting cell proliferation and lymph node metastasis in urothelial bladder cancer (PMID: 37573356).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ANAPC11 antibody 15065-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
