APC4 Polyclonal antibody

APC4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14129-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

ANAPC4, APC4, Cyclosome subunit 4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHC

WB analysis using 14129-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14129-1-AP (APC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human brain using 14129-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 14129-1-AP (APC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 14129-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14129-1-AP (APC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 14129-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14129-1-AP (APC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	HEK-293 cells, human brain tissue, Hela cells, MCF-7 cells, HIN/3T3 cells
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

14129-1-AP targets APC4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5225 Product name: Recombinant human ANAPC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-343 aa of BC059383 Sequence: MLRFPTCFPSFRVVGEKQLPQEIIFLVWSPKRDLIALANTAGEVLLHRLASFHRVWSFPPNENTGKEVTCLAWRPDGKLLAFALADTKKIVLCDVEKPESLHSFSVEAPVSCMHWMEVTVESSVLTSFYNAEDESNLLLPKLPTLPKNYSNTSKIFSEENSDEIIKLLGDVRLNILVLGGSSGFIELYAYGMFKIARVTGIAGTCLALCLSSDLKSLSVVTEVSTNGASEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSLTCMCEAWEEILMQMDSRLTKFVQEKNTTTSVQDEFMHLLLWGKASAELQTLLMNQLTVKGLKKLGQSIESSYSS Predict reactive species
Full Name	anaphase promoting complex subunit 4
Calculated Molecular Weight	92 kDa
Observed Molecular Weight	92 kDa, 63 kDa
GenBank Accession Number	BC059383
Gene Symbol	APC4
Gene ID (NCBI)	29945
RRID	AB_10640671
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UJX5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for APC4 antibody 14129-1-AP	Download protocol
WB protocol for APC4 antibody 14129-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Adv Sci (Weinh) CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC Authors - Qi Li View Article
mouse	IF	PLoS Pathog A murine cytomegalovirus cell cycle regulator (m54.5p) evolved within the conserved viral DNA polymerase gene. Authors - Yan Zheng View Article