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ANGPTL1 Polyclonal antibody

ANGPTL1 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 14709-1-AP

Synonyms

ANG 3, ANG3, Angiopoietin 3, angiopoietin like 1, Angiopoietin like protein 1, Angiopoietin related protein 1, ANGPT3, ANGPTL1, AngY, ARP1, KIAA0351, UNQ162, UNQ162/PRO188

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ANGPTL1 Polyclonal antibody (14709-1-AP)

    WB analysis of HeLa using 14709-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14709-1-AP (ANGPTL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using ANGPTL1 Polyclonal antibody (14709-1-AP)

    WB analysis of human heart using 14709-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14709-1-AP (ANGPTL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using ANGPTL1 Polyclonal antibody (14709-1-AP)

    WB analysis of BxPC-3 using 14709-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14709-1-AP (ANGPTL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ANGPTL1 Polyclonal antibody (14709-1-AP)

    WB analysis of HEK-293 using 14709-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14709-1-AP (ANGPTL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ANGPTL1 Polyclonal antibody (14709-1-AP)

    IHC staining of human ovary tumor using 14709-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14709-1-AP (ANGPTL1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ANGPTL1 Polyclonal antibody (14709-1-AP)

    IHC staining of human ovary tumor using 14709-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14709-1-AP (ANGPTL1 antibody) at dilution of 1:50 (under 10x lens).

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14709-1-AP targets ANGPTL1 in WB,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANGPTL1 fusion protein Ag6403
Full Name angiopoietin-like 1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC050640
Gene Symbol ANGPTL1
Gene ID (NCBI) 9068
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiopoietin-like protein (Angptl) 1, also known as angiopoietin-related protein 1 (ARP1) and angioarrestin, belongs to the angiopoietin-like family of proteins (Angptls). It is a secretory glycoprotein that shares two basic structural motifs with the angiopoietin family: an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain. Angptl1 is widely expressed in adult tissues with mRNA levels highest in highly vascularized tissues. It is related to development of the connective tissue and cartilage. This protein was found to be a secretory protein that does not act as an endothelial cell mitogen in vitro.

Publications

SpeciesApplicationTitle
hamsterWB

Protein Cell

Dynamic roles of angiopoietin-like proteins 1, 2, 3, 4, 6 and 7 in the survival and enhancement of ex vivo expansion of bone-marrow hematopoietic stem cells.

Authors - Akhter Shahina S
View Article