Filter: AllWBIHCIF/ICC

WB analysis using 24203-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24203-1-AP (ANKRD55 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 24203-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 24203-1-AP (ANKRD55 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 24203-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 24203-1-AP (ANKRD55 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human cervical cancer using 24203-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24203-1-AP (ANKRD55 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cervical cancer using 24203-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24203-1-AP (ANKRD55 Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 24203-1-AP
Immunofluorescent analysis of HeLa cells using 24203-1-AP (ANKRD55 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ANKRD55 Polyclonal antibody

ANKRD55 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24203-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ankyrin repeat domain-containing protein 55

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, L02 cells, mouse spleen tissue, mouse small intestine tissue, mouse thymus tissue
Positive IHC detected in	human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24203-1-AP targets ANKRD55 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21271 Product name: Recombinant human ANKRD55 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-326 aa of BC106914 Sequence: MDSNLRDINESTPLAYALYCGHTACVKLLSQESRTEPTRPPPSQSSRPQKKERRFNVLNQIFCKNKKEEQRAHQKDPSRDRYREEDTSEVNDIITTFDSIVGTNCQEQPGDQVAMVEFKKKTSDNSKYLLPEKKPLARKGLPPIRTQSLPPITLGNNFLTASHRATSHAGLSSAPHHMAQRSQKSRSEQDLLNNRTGCQMLLDNPWKSDSNQVFSYKVWTVSSSDKLLDRLLSVRPGHQEVSVPPHLRHLHNPSSGQNFQHLSPNRHKIRDLPFTRNNLAPLPDQKFLSGEPLRTNRVLPAIPSQRRHSTAAEESEHSANPTSDEN Predict reactive species
Full Name	ankyrin repeat domain 55
Calculated Molecular Weight	614 aa, 68 kDa
Observed Molecular Weight	68 kDa
GenBank Accession Number	BC106914
Gene Symbol	ANKRD55
Gene ID (NCBI)	79722
RRID	AB_2879456
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q3KP44
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKRD55 also named as Ankyrin repeat domain containing protein 55 is a 614 amino acid protein, which contains 9 ANK repeat. ANKRD55 may exist three alternatively spliced isoforms and belongs to ankyrin family. The function of ANKRD55 is still non-known and need to be further studied. ANKRD55 is a critical regulator of TCR signaling and a key regulator of T cell inflammation in multiple sclerosis (PMID: 41090353).

Protocols

Product Specific Protocols
IF protocol for ANKRD55 antibody 24203-1-AP	Download protocol
IHC protocol for ANKRD55 antibody 24203-1-AP	Download protocol
WB protocol for ANKRD55 antibody 24203-1-AP	Download protocol