Tested Applications
| Positive WB detected in | BxPC-3 cells, Caco-2 cells, HeLa cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20447-1-AP targets ANKZF1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14285 Product name: Recombinant human ANKZF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 377-726 aa of BC000238 Sequence: TEEEIRKICRDEKEALGQNEESPKQGSGSEGEDGFQVELELVELTVGTLDLCESEVLPKRRRRKRNKKEKSRDQEAGAHRTLLQQTQEEEPSTQSSQAVAAPLGPLLDEAKAPGQPELWNALLAACRAGDVGVLKLQLAPSPADPRVLSLLSAPLGSGGFTLLHAAAAAGRGSVVRLLLEAGADPTVQDSRARPPYTVAADKSTRNEFRRFMEKNPDAYDYNKAQVPGPLTPEMEARQATRKREQKAARRQREEQQQRQQEQEEREREEQRRFAALSDREKRALAAERRLAAQLGAPTSPIPDSAIVNTRRCWSCGASLQGLTPFHYLDFSFCSTRCLQDHRRQAGRPSS Predict reactive species |
| Full Name | ankyrin repeat and zinc finger domain containing 1 |
| Calculated Molecular Weight | 726 aa, 81 kDa |
| Observed Molecular Weight | 81 kDa |
| GenBank Accession Number | BC000238 |
| Gene Symbol | ANKZF1 |
| Gene ID (NCBI) | 55139 |
| RRID | AB_2878688 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H8Y5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ANKZF1, the mammalian ortholog of yeast Vms1, functions in the ribosome quality control (RQC) pathway as a tRNA hydrolase that recycles nascent peptides from stalled ribosomes, and it also counterbalances Rqc2‑mediated CAT‑tailing, thereby limiting the import of CAT‑tailed mitochondrial precursor proteins and reducing their aggregation within mitochondria. Under oxidative stress, both Vms1 and ANKZF1 localize to mitochondria and recruit Cdc48/VCP to facilitate mitochondria‑associated degradation (MAD), although the physiological requirement for this mitochondrial targeting remains unclear. Notably, recent studies in yeast reveal that VMS1 deletion aggravates proteotoxicity in the mitochondrial matrix, highlighting its essential protective role during mitochondrial stress.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ANKZF1 antibody 20447-1-AP | Download protocol |
| WB protocol for ANKZF1 antibody 20447-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Acta Neuropathol Commun Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease | ||
Nucleic Acids Res Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats | ||
Oncol Rep Overexpression of ZNF169 promotes the growth and proliferation of colorectal cancer cells via the upregulation of ANKZF1 | ||
bioRxiv Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats | ||
EMBO J The coordinated action of UFMylation and the RQC pathways clears arrested polypeptides at the ER.
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