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Tested Applications
| Positive WB detected in | mouse liver tissue, mouse lung tissue, mouse pancreas tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 12 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
19495-1-AP targets Aldehyde oxidase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Reactivity | human, mouse, rat, canine |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | aldehyde oxidase 1 |
| Calculated Molecular Weight | 148 kDa |
| Observed Molecular Weight | 148 kDa |
| GenBank Accession Number | NM_001159 |
| Gene Symbol | AOX1 |
| Gene ID (NCBI) | 316 |
| RRID | AB_10640579 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q06278 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Aldehyde oxidase antibody 19495-1-AP | Download protocol |
| IHC protocol for Aldehyde oxidase antibody 19495-1-AP | Download protocol |
| IP protocol for Aldehyde oxidase antibody 19495-1-AP | Download protocol |
| WB protocol for Aldehyde oxidase antibody 19495-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine Integrated metabolomics and mass spectrometry imaging analysis reveal the efficacy and mechanism of Huangkui capsule on type 2 diabetic nephropathy | ||
EMBO Rep Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice. | ||
Oncogene Epigenetic loss of AOX1 expression via EZH2 leads to metabolic deregulations and promotes bladder cancer progression.
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Free Radic Biol Med PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase. | ||
Front Immunol Insight of a Metabolic Prognostic Model to Identify Tumor Environment and Drug Vulnerability for Lung Adenocarcinoma. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH MJ (Verified Customer) (05-27-2025) | Clean signal at 148kDA on rat brain lysate using 10% gel on 0.45um PVDF diluted in Hikari Signal Enhancer
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