Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15722-1-AP targets AP2A2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8442 Product name: Recombinant human AP2A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 590-939 aa of BC006155 Sequence: STDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTSAVSTPSPSADLLGLGAAPPAPAGPPPSSGGSGLLVDVFSDSASVVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICSDDLQPNLNLQTKPVDPTVEGGAQVQQVVNIECVSDFTEAPVLNIQFRYGGTFQNVSVQLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEVTKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKEAVSQRLCELLSAQF Predict reactive species |
| Full Name | adaptor-related protein complex 2, alpha 2 subunit |
| Calculated Molecular Weight | 939 aa, 104 kDa |
| Observed Molecular Weight | 104 kDa |
| GenBank Accession Number | BC006155 |
| Gene Symbol | AP2A2 |
| Gene ID (NCBI) | 161 |
| RRID | AB_2878175 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94973 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AP2A2, also named as ADTAB, CLAPA2, HIP9, HYPJ, or KIAA0899, is a 939 amino acid protein, which belongs to the adaptor complexes large subunit family. AP2A2 localizes in the membrane and is a component of the adaptor protein complex 2. AP2 adaptor complex linking clathrin to receptors in coated vesicles, clathrin-associated protein complexes are believed to interact with cytoplasmic domains of transmembrane proteins leading to their selection and concentration. AP2A2 is invovled into cellular trafficking transport.
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Emerg Microbes Infect CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis | ||
Proteomics Proteomics Reveals AP-2 Complex Depletion Suppressing Listeria monocytogenes Intracellular Replication |
