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WB analysis of PC-3 cells using 60100-1-Ig
WB result of APOBEC3G antibody (60100-1-Ig, 1:1000) with si-Control and si-APOBEC3G transfected PC-3 cells.
WB analysis of A375 using 60100-1-Ig
A375 cells were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 60100-1-Ig
A375 cells were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 60100-1-Ig
PC-3 cells were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 60100-1-Ig
human testis tissue were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 60100-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 60100-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 60100-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 60100-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 40x lens).
IF Staining of C6 using 60100-1-Ig
Immunofluorescent analysis of C6 cells using 60100-1-Ig (APOBEC3G antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

Featured Product
KD/KO Validated

APOBEC3G Monoclonal antibody

Name: APOBEC3G Monoclonal antibody
Brand: Proteintech
SKU: 60100-1-Ig

APOBEC3G Monoclonal Antibody for WB, IF, IHC, ELISA

Cat No. 60100-1-Ig

Clone No.5G10A8

Publications(2)

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

APOBEC related protein, APOBEC related protein 9, APOBEC3G, ARCD, ARP 9, ARP9, bK150C2.7, CEM 15, CEM15, MDS019

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A375 cells, PC-3 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	C6 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

60100-1-Ig targets APOBEC3G in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag7770 Product name: Recombinant human APOBEC3G protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 84-384 aa of BC024268 Sequence: QEYEVTWYISWSPCTKCTRDMATFLAEDPKVTLTIFVARLYYFWDPDYQEALRSLCQKRDGPRATMKIMNYDEFQHCWSKFVYSQRELFEPWNNLPKYYILLHIMLGEILRHSMDPPTFTFNFNNEPWVRGRHETYLCYEVERMHNDTWVLLNQRRGFLCNQAPHKHGFLEGRHAELCFLDVIPFWKLDLDQDYRVTCFTSWSPCFSCAQEMAKFISKNKHVSLCIFTARIYDDQGRCQEGLRTLAEAGAKISIMTYSEFKHCWDTFVDHQGCPFQPWDGLDEHSQDLSGRLRAILQNQEN Predict reactive species
Full Name	apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
Calculated Molecular Weight	46 kDa
Observed Molecular Weight	46 kDa
GenBank Accession Number	BC024268
Gene Symbol	APOBEC3G
Gene ID (NCBI)	60489
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9HC16
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APOBEC3G((apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G,also known as CEM15), a member of the APOBEC family of cytidine deaminase-editing enzymes, is a potent DNA mutator that can be packaged into budding retroviruses.HA-tagged APOBEC3G can be expressed in 293T cells, which do not express APOBEC3G endogenously,in addition 22- and 24-kDa proteolytic degradation fragments can be detected by western blot(PMID:14672928). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for APOBEC3G antibody 60100-1-Ig	Download protocol
IHC protocol for APOBEC3G antibody 60100-1-Ig	Download protocol
WB protocol for APOBEC3G antibody 60100-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Rep Hepatitis B virus X protein is capable of down-regulating protein level of host antiviral protein APOBEC3G. Authors - Ruidong Chen View Article
human	IHC	Int J Clin Exp Med APOBEC3G expression is correlated with poor prognosis in colon carcinoma patients with hepatic metastasis. Authors - Huanrong Lan View Article