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APOBEC3G Monoclonal antibody

APOBEC3G Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF

Conjugate

Unconjugated

CloneNo.

5G10A8

Cat no : 60100-1-Ig

Synonyms

APOBEC related protein, APOBEC related protein 9, APOBEC3G, ARCD, ARP 9, ARP9, bK150C2.7, CEM 15, CEM15, MDS019

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using APOBEC3G Monoclonal antibody (60100-1-Ig)

    WB analysis of PC-3 cells using 60100-1-Ig

    WB result of APOBEC3G antibody (60100-1-Ig, 1:1000) with si-Control and si-APOBEC3G transfected PC-3 cells.

  • Western Blot (WB) analysis of A375 cells using APOBEC3G Monoclonal antibody (60100-1-Ig)

    WB analysis of A375 using 60100-1-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using APOBEC3G Monoclonal antibody (60100-1-Ig)

    WB analysis of human testis using 60100-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using APOBEC3G Monoclonal antibody (60100-1-Ig)

    WB analysis of A375 using 60100-1-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using APOBEC3G Monoclonal antibody (60100-1-Ig)

    WB analysis of PC-3 using 60100-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APOBEC3G Monoclonal antibody (60100-1-Ig)

    IHC staining of human breast cancer using 60100-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APOBEC3G Monoclonal antibody (60100-1-Ig)

    IHC staining of human breast cancer using 60100-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APOBEC3G Monoclonal antibody (60100-1-Ig)

    IHC staining of human breast cancer using 60100-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APOBEC3G Monoclonal antibody (60100-1-Ig)

    IHC staining of human breast cancer using 60100-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using APOBEC3G Monoclonal antibody (60100-1-Ig)

    IF Staining of C6 using 60100-1-Ig

    Immunofluorescent analysis of C6 cells using 60100-1-Ig (APOBEC3G antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

Tested Applications

Positive WB detected inA375 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60100-1-Ig targets APOBEC3G in WB, IHC, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen APOBEC3G fusion protein Ag7770
Full Name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC024268
Gene Symbol APOBEC3G
Gene ID (NCBI) 60489
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APOBEC3G((apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G,also known as CEM15), a member of the APOBEC family of cytidine deaminase-editing enzymes, is a potent DNA mutator that can be packaged into budding retroviruses.HA-tagged APOBEC3G can be expressed in 293T cells, which do not express APOBEC3G endogenously,in addition 22- and 24-kDa proteolytic degradation fragments can be detected by western blot(PMID:14672928). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for APOBEC3G antibody 60100-1-IgDownload protocol
IHC protocol for APOBEC3G antibody 60100-1-IgDownload protocol
IF protocol for APOBEC3G antibody 60100-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

Hepatitis B virus X protein is capable of down-regulating protein level of host antiviral protein APOBEC3G.

Authors - Ruidong Chen
View Article
humanIHC

Int J Clin Exp Med

APOBEC3G expression is correlated with poor prognosis in colon carcinoma patients with hepatic metastasis.

Authors - Huanrong Lan
View Article