ARF5-Specific Antibody
Rabbit Polyclonal
Catalog number: 20227-1-AP
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Applications
| Tested applications | ELISA, WB, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | mouse colon tissue, COLO 320 cells, mouse brain tissue, human testis tissue |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:500-1:5000 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
mouse colon tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP(ARF5-Specific Antibody) at dilution of 1:1500
Immunofluorescent analysis of HepG2 cells, using ARF5 antibody 20227-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Peptide | Full name | ADP-ribosylation factor 5 |
|
Calculated molecular weight |
21kd |
Observed molecular
weight |
21kd |
|
GenBank accession number |
NM_001662 | Gene ID (NCBI) | 381 |
| Gene symbol | ARF5 | Synonyms |
Background
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. (PMID: 7759471, PMID: 16042562). Their function are not fully described.The antibody is specific to ARF5. |
