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ARHGAP1 Polyclonal antibody

ARHGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11169-1-AP
Publications (1)
Review Product
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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

p50-RhoGAP, p50rhoGAP, p50 RhoGAP, GTPase-activating protein rhoGAP, CDC42GAP

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
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  • Western Blot (WB) analysis of RAW 264.7 cells using ARHGAP1 Polyclonal antibody (11169-1-AP)

    WB analysis of RAW 264.7 using 11169-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of PC-12 cells using ARHGAP1 Polyclonal antibody (11169-1-AP)

    WB analysis of PC-12 using 11169-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of human duodenum tissue using ARHGAP1 Polyclonal antibody (11169-1-AP)

    WB analysis of human duodenum using 11169-1-AP

    human duodenum tissue were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of human pancreas tissue using ARHGAP1 Polyclonal antibody (11169-1-AP)

    WB analysis of human pancreas using 11169-1-AP

    human pancreas tissue were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of human heart tissue using ARHGAP1 Polyclonal antibody (11169-1-AP)

    WB analysis of human heart using 11169-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human pancreas tissue using ARHGAP1 Polyclonal antibody (11169-1-AP)

    IHC staining of human pancreas using 11169-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:200 (under 10x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human pancreas tissue using ARHGAP1 Polyclonal antibody (11169-1-AP)

    IHC staining of human pancreas using 11169-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:200 (under 40x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ARHGAP1 Polyclonal antibody (11169-1-AP)

    IHC staining of human pancreas cancer using 11169-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11169-1-AP (ARHGAP1 antibody) at dilution of 1:50 (under 10x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARHGAP1 Polyclonal antibody (11169-1-AP)

    IF Staining of HeLa using 11169-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARHGAP1 antibody (11169-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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Tested Applications

Positive WB detected inRAW 264.7 cells, human duodenum tissue, human heart tissue, human pancreas tissue, PC-12 cells
Positive IHC detected inhuman pancreas tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11169-1-AP targets ARHGAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1651

Product name: Recombinant human ARHGAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 91-440 aa of BC018118

Sequence: CRMPPSHQLDHSKLLGYLKHTLDQYVESDYTLLYLHHGLTSDNKPSLSWLRDAYREFDRKYKKNIKALYIVHPTMFIKTLLILFKPLISFKFGQKIFYVNYLSELSEHVKLEQLGIPRQVLKYDDFLKSTQKSPATAPKPMPPRPPLPNQQFGVSLQHLQEKNPEQEPIPIVLRETVAYLQAHALTTEGIFRRSANTQVVREVQQKYNMGLPVDFDQYNELHLPAVILKTFLRELPEPLLTFDLYPHVVGFLNIDESQRVPATLQVLQTLPEENYQVLRFLTAFLVQISAHSDQNKMTNTNLAVVFGPNLLWAKDAAITLKAINPINTFTKFLLDHQGELFPSPDPSGL

Predict reactive species
Full Name Rho GTPase activating protein 1
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC018118
Gene Symbol ARHGAP1
Gene ID (NCBI) 392
RRIDAB_2289862
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07960
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for ARHGAP1 antibody 11169-1-APDownload protocol
IHC protocol for ARHGAP1 antibody 11169-1-APDownload protocol
WB protocol for ARHGAP1 antibody 11169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The ARHGAP1 Polyclonal antibody (Cat# 11169-1-AP) has 1 total citation. It appears in Nature Communications (IF 18.1), in a 2025 study on endosomal proximity proteome mapping that identified Retromer as a hub for RAB GTPase regulation. The research field encompasses cell biology, intracellular trafficking, and GTPase signaling. The antibody was used in WB and IF applications.

Automatically generated from the 1 publication citations by Able AI.
SpeciesApplicationTitle
WB,IF

Nat Commun

Mapping of endosomal proximity proteomes reveals Retromer as a hub for RAB GTPase regulation

Authors - Carlos Antón-Plágaro
View Article
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