Tested Applications
| Positive WB detected in | Jurkat cells, Ramos cells, THP-1 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14349-1-AP targets ARHGAP25 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5691 Product name: Recombinant human ARHGAP25 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-347 aa of BC039591 Sequence: MSLGQSACLFLSIARSRSVMTGEQMAAFHPSSTPNPLERPIKMGWLKKQRSIVKNWQQRYFVLRAQQLYYYKDEEDTKPQGCMYLPGCTIKEIATNPEEAGKFVFEIIPASWDQNRMGQDSYVLMASSQAEMEEWVKFLRRVAGTPCGAVFGQRLDETVAYEQKFGPHLVPILVEKCAEFILEHGRNEEGIFRLPGQDNLVKQLRDAFDAGERPSFDRDTDVHTVASLLKLYLRDLPEPVVPWSQYEGFLLCGQLTNADEAKAQQELMKQLSILPRDNYSLLSYICRFLHEIQLNCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDHEVLF Predict reactive species |
| Full Name | Rho GTPase activating protein 25 |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC039591 |
| Gene Symbol | ARHGAP25 |
| Gene ID (NCBI) | 9938 |
| RRID | AB_2059273 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42331 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ARHGAP25 (Rho GTPase-activating protein 25) also known as KAIA0053, belongs to a family of Rho GTPase-modulating proteins which are implicated in actin remodeling,cell polarity, and cell migration. ARHGAP25 has a key role in the pathomechanism of autoantibody-induced arthritis in which it regulates inflammation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ARHGAP25 antibody 14349-1-AP | Download protocol |
| IHC protocol for ARHGAP25 antibody 14349-1-AP | Download protocol |
| IP protocol for ARHGAP25 antibody 14349-1-AP | Download protocol |
| WB protocol for ARHGAP25 antibody 14349-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ARHGAP25 Polyclonal antibody (Cat# 14349-1-AP) has 3 citations published in Carcinogenesis, Journal of Molecular Histology, and American Journal of Translational Research. The cited studies span cancer biology (breast cancer and osteosarcoma progression) and gastroenterology/hepatology (irritable bowel syndrome–NAFLD comorbidity biomarkers), with applications in Western blot and IHC across human and mouse samples.
| Species | Application | Title |
|---|---|---|
Carcinogenesis ARHGAP25 suppresses the development of breast cancer by an ARHGAP25/Wnt/ASCL2 feedback loop
| ||
J Mol Histol Tenascin-C-enriched extracellular vesicles contribute to osteosarcoma progression via regulation of the angiotensin II receptor type 1 pathway. | ||
Am J Transl Res A set of downregulated pleiotropic genes are possible multi-omics biomarkers underlying the irritable bowel syndrome-non-alcoholic fatty liver disease comorbidity. |
