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WB analysis using 20506-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 20506-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 20506-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20506-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20506-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 20506-1-AP
IP result of anti-ARMC10 (IP:20506-1-AP, 4ug; Detection:20506-1-AP 1:500) with A431 cells lysate 1600ug.
IHC staining of human placenta using 20506-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 20506-1-AP (ARMC10 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 20506-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 20506-1-AP (ARMC10 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 20506-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ARMC10 antibody (20506-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
IF Staining of A431 using 20506-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARMC10 antibody (20506-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
IF Staining of A431 using 20506-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 20506-1-AP (ARMC10 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

ARMC10 Polyclonal antibody

Name: ARMC10 Polyclonal antibody
Brand: Proteintech
SKU: 20506-1-AP

ARMC10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20506-1-AP

Publications(5)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

armadillo repeat containing 10, Armadillo repeat-containing protein 10, PNAS 112, PSEC0198, SVH

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse heart tissue, HEK-293 cells, HepG2 cells, mouse brain tissue, mouse liver tissue, rat heart tissue
Positive IP detected in	A431 cells
Positive IHC detected in	human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 4 publications below
IHC	See 2 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

20506-1-AP targets ARMC10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14313 Product name: Recombinant human ARMC10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 47-308 aa of BC003586 Sequence: EDLTDGSYDDVLNAEQLQKLLYLLESTEDPVIIERALITLGNNAAFSVNQAIIRELGGIPIVANKINHSNQSIKEKALNALNNLSVNVENQIKIKIYISQVCEDVFSGSLNSAVQLAGLTLLTNMTVTNDHQHMLHSYITDLFQVLLTGNGNTKVQVLKLLLNLSENPAMTEGLLRAQVDSSFLSLYDSHVAKEILLRVLTLFQNIKNCLKIEGHLAVQPTFTEGSLFFLLHGEECAQKIRALVDHHDAEVKEKVVTIIPKI Predict reactive species
Full Name	armadillo repeat containing 10
Calculated Molecular Weight	343 aa, 38 kDa
Observed Molecular Weight	31-37 kDa
GenBank Accession Number	BC003586
Gene Symbol	ARMC10
Gene ID (NCBI)	83787
RRID	AB_10696176
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N2F6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for ARMC10 antibody 20506-1-AP	Download protocol
IHC protocol for ARMC10 antibody 20506-1-AP	Download protocol
IP protocol for ARMC10 antibody 20506-1-AP	Download protocol
WB protocol for ARMC10 antibody 20506-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Cell Death Dis The Armc10/SVH gene: genome context, regulation of mitochondrial dynamics and protection against Aβ-induced mitochondrial fragmentation. Authors - R Serrat View Article
human	WB	Heliyon Prognostic value and immune infiltration of ARMC10 in pancreatic adenocarcinoma via integrated bioinformatics analyses Authors - Tian-Hao Li KD Validated View Article
human	WB,IF	J Cell Mol Med ARMC10 regulates mitochondrial dynamics and affects mitochondrial function via the Wnt/β-catenin signalling pathway involved in ischaemic stroke Authors - Yanyang Huang KD Validated View Article
human	IHC	BMC Cancer Comprehensive analysis of the expression and prognostic value of ARMCs in pancreatic adenocarcinoma Authors - Guanxiang Zhuo View Article
human	WB,IHC,IF	Mol Carcinog ARMC10 Drives Glioblastoma Progression Through Activating Notch Pathway Authors - Bin Feng KD Validated View Article