ASB9 Polyclonal antibody

ASB9 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11952-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

ASB-9, ASB 9

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHC

WB analysis of human testis using 11952-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 11952-1-AP (ASB9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 11952-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11952-1-AP (ASB9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 11952-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11952-1-AP (ASB9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	human testis tissue, HepG2 cells
Positive IHC detected in	human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

11952-1-AP targets ASB9 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2554 Product name: Recombinant human ASB9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-252 aa of BC001244 Sequence: MDGKQGGMDGSKPAGPRDFPGIRLLSNPLMGDAVSDWSPMHEAAIHGHQLSLRNLISQGWAVNIITADHVSPLHEACLGGHLSCVKILLKHGAQVNGVTADWHTPLFNACVSGSWDCVNLLLQHGASVQPESDLASPIHEAARRAYGGNIDHKISHLGTPLYLACENQQRACVKKLLESGADVNQGKGQDSPLHAVARTASEELACLLMDFGADTQAKNAEGKRPVELVPPESPLAQLFLEREGASLPKPKP Predict reactive species
Full Name	ankyrin repeat and SOCS box-containing 9
Calculated Molecular Weight	252 aa, 28 kDa, 32 kDa
Observed Molecular Weight	28-32 kDa
GenBank Accession Number	BC001244
Gene Symbol	ASB9
Gene ID (NCBI)	140462
RRID	AB_10666996
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96DX5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for ASB9 antibody 11952-1-AP	Download protocol
WB protocol for ASB9 antibody 11952-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	IHC	Molecules Identification of Key Candidate Genes Involved in the Progression of Idiopathic Pulmonary Fibrosis. Authors - Yu Cui View Article
		Oncol Rep HMQ‑T‑F2 suppresses migration of the human cervical cancer HeLa cells by reversing EMT via the PI3K/Akt signaling pathway. Authors - Bingling Dai View Article