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  • KD/KO Validated

ASH2L Polyclonal antibody

ASH2L Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 12331-1-AP

Synonyms

ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2



Tested Applications

Positive WB detected inCOS-7 cells, COLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12331-1-AP targets ASH2L in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity human, mouse, monkey
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASH2L fusion protein Ag3008
Full Name ash2 (absent, small, or homeotic)-like (Drosophila)
Calculated Molecular Weight 628 aa, 69 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC015936
Gene Symbol ASH2L
Gene ID (NCBI) 9070
RRIDAB_2059837
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ASH2L antibody 12331-1-APDownload protocol
IHC protocol for ASH2L antibody 12331-1-APDownload protocol
IP protocol for ASH2L antibody 12331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.

Authors - Esteban A Orellana
humanWB

Nat Commun

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Authors - Gongwei Wu
humanWB,IHC,IF,IP,ChIP

Cancer Sci

ASH2L is involved in promotion of endometrial cancer progression via upregulation of PAX2 transcription.

Authors - Kai Zeng
  • KD Validated
mouseWB,IP

Mol Neurobiol

WDR5-HOTTIP Histone Modifying Complex Regulates Neural Migration and Dendrite Polarity of Pyramidal Neurons via Reelin Signaling.

Authors - Minhan Ka