Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23894-1-AP targets ATAD2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20947 Product name: Recombinant human ATAD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC113656 Sequence: MVVLRSSLELHNHSAASATGSLDLSSDFLSLEHIGRRRLRSAGAAQKKPAATTAKAGDGSSVKEVETYHRTRALRSLRKDAQNSSDSSFEKNVEITEQLANGRHFTRQLARQQADKKKEEHREDKVIPVTRSLRARNIVQSTEHLHEDNG Predict reactive species |
| Full Name | ATPase family, AAA domain containing 2 |
| Calculated Molecular Weight | 1390 aa, 159 kDa |
| Observed Molecular Weight | 159 kDa |
| GenBank Accession Number | BC113656 |
| Gene Symbol | ATAD2 |
| Gene ID (NCBI) | 29028 |
| RRID | AB_2879352 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6PL18 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATAD2 is one of them encoding a conserved factor harbouring an AAA type ATPase domain and a bromodomain. ATAD2 controls chromatin dynamics, genome transcriptional activities and apoptotic cell response. This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATAD2 antibody 23894-1-AP | Download protocol |
| IP protocol for ATAD2 antibody 23894-1-AP | Download protocol |
| WB protocol for ATAD2 antibody 23894-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Cell Rep Proteomic profiling reveals distinct phases to the restoration of chromatin following DNA replication | ||
Biomed Pharmacother Silencing METTL3 inhibits the proliferation and invasion of osteosarcoma by regulating ATAD2. | ||
Mol Cell Biol Mutual Balance of Histone Deacetylases 1 and 2 and the Acetyl Reader ATAD2 Regulates the Level of Acetylation of Histone H4 on Nascent Chromatin of Human Cells.
| ||
Biochem Biophys Res Commun ATAD2 drives colorectal cancer progression by regulating TRIM25 expression via a positive feedback loop with E2F transcriptional factors.
| ||
iScience Genome-wide survey of D/E repeats in human proteins uncovers their instability and aids in identifying their role in the chromatin regulator ATAD2 | ||
FEBS Lett The E3 ubiquitin ligase UBR5 promotes ubiquitylation and degradation of the chromatin regulator ATAD2.
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