ATF4 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | A431 cells, HEK-293 cells, SGC-7901 cells, DU 145 cells |
| Positive IHC detected in | human breast cancer |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:50-1:200 |
IP = Immunoprecipitation
IP result of anti-ATF4 (10835-1-AP for IP and Detection) with HEK-293 cell lysate.
A431 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP(ATF4 Antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human breast cancer using 10835-1-AP(ATF4 Antibody) at Dilution 1:100 (under 10x lens)
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | ATF4 fusion protein ag1279 | Full name | activating transcription factor 4 (tax-responsive enhancer element B67) |
|
Calculated molecular weight |
39kd |
Observed molecular
weight |
50-55 kDa, 35-38 kDa |
|
GenBank accession number |
BC022088 | Gene ID (NCBI) | 468 |
| Gene symbol | ATF4 | Synonyms | CREB-2; CREB2; TAXREB67; TXREB |
ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. Endogenous ATF4 protein has a molecular mass of 50kd. [PMID: 17726049]. This antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen. |
