ATG5 Antibody
Rabbit Polyclonal
Catalog number: 10181-2-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse, rat |
| Positive WB detected in | mouse kidney tissue, human brain tissue |
| Positive IHC detected in | human colon cancer |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IF result of anti-ATG5 (10181-2-AP) with E15 mouse cortical neurons.
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10181-2-AP(ATG5 Antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human colon cancer using 10181-2-AP(ATG5 Antibody) at Dilution 1:50 (under 10x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | ATG5 fusion protein ag0214 | Full name | ATG5 autophagy related 5 homolog (S. cerevisiae) |
|
Calculated molecular weight |
32KD |
Observed molecular
weight |
50-55kd |
|
GenBank accession number |
BC002699 | Gene ID (NCBI) | 9474 |
| Gene symbol | ATG5 | Synonyms | APG5; APG5-LIKE; APG5L; ASP; hAPG5 |
Background
ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of full protein. It can also recognize the ATG5-ATG12 complex(55kd). |
