ATG5 Antibody
Mouse Monoclonal
Catalog number: 60061-1-Ig
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Applications
| Tested applications | ELISA, WB |
| Cited applications | |
| Species specificity | human, mouse; other species not tested. |
| Cited species | |
| Positive WB detected in | Colo320 cells |
| Recommended dilution | WB: 1:200-1:2000 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Colo320 cells were subjected to SDS PAGE followed by western blot with 60061-1-Ig(ATG5 Antibody) at dilution of 1:500
Product Information
| Source | Mouse | Purification method | Caprylic acid/ammonium sulfate precipitation |
| Isotype | IgM | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | ATG5 fusion protein ag0214 | Full name | ATG5 autophagy related 5 homolog (S. cerevisiae) |
|
Calculated molecular weight |
32KD |
Observed molecular
weight |
34kd |
|
GenBank accession number |
BC002699 | Gene ID (NCBI) | 9474 |
| Gene symbol | ATG5 | Synonyms | APG5; APG5-LIKE; APG5L; ASP; hAPG5 |
Background
ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. |
