Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 2 publications below |
Product Information
25725-1-AP targets CARNS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22812 Product name: Recombinant human ATPGD1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 443-827 aa of BC036557 Sequence: MTEHRRDEENARLLAELVRARGLKLDGCFSYWDDCLVLTALLCQELGLPCSSPAALRLAKQKSLTQLHLLHHHGPPWPAPSLHAVPCCPLESEADVERAVHQVPLPGVMKLEFGAGAVGVRLVEDAPQCHEHFSRITRDLQGEADHPGIGLGWGNAMLLMEFVEGTEHDVDLVLFGGRLLAAFVSDNGPTRLPGFTETAACMPTGLAPEQEAQMVQAAFRCCLGCGLLDGVFNVELKLTGAGPRLIEINPRMGGFYLRDWILELYGVDLLLAAVMVACGLRPALPTRPRARGHLVGVMCLVSQHLQALSSTASRETLQALHDRGLLRLNLLEEALVPGEYEEPYCSVACAGPSPTEARLRLLGLCQGLGIDGPSYPVAHFLSHFK Predict reactive species |
| Full Name | ATP-grasp domain containing 1 |
| Calculated Molecular Weight | 827 aa, 88 kDa |
| Observed Molecular Weight | 88-103 kDa |
| GenBank Accession Number | BC036557 |
| Gene Symbol | CARNS1 |
| Gene ID (NCBI) | 57571 |
| RRID | AB_2880211 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A5YM72 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CARNS1, located at human chromosome 11q13.2, encodes carnosine synthase 1 belonging to the ATP-grasp ATPase family (EC 6.3.2.11). It mediates ATP-dependent synthesis of carnosine in skeletal muscle and homocarnosine in the central nervous system, two vital histidine-containing dipeptides with antioxidant and pH-buffering effects. This gene is highly conserved across vertebrates and preferentially expressed in brain and muscle tissues. Aberrant CARNS1 expression disturbs dipeptide homeostasis, linking to aging-related sensory decline and tumor progression via impaired carnosine-mediated tumor suppression. (PMID: 20097752)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CARNS1 antibody 25725-1-AP | Download protocol |
| WB protocol for CARNS1 antibody 25725-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Part Fibre Toxicol Aryl hydrocarbon receptor activation-mediated vascular toxicity of ambient fine particulate matter: contribution of polycyclic aromatic hydrocarbons and osteopontin as a biomarker. | ||
Transl Stroke Res Nicotine Exposure Along with Oral Contraceptive Treatment in Female Rats Exacerbates Post-cerebral Ischemic Hypoperfusion Potentially via Altered Histamine Metabolism. | ||
Neurochem Res Carnosine Reduces Oxidative Stress and Reverses Attenuation of Righting and Postural Reflexes in Rats with Thioacetamide-Induced Liver Failure. | ||
