KD/KO Validated

AUH Polyclonal antibody

AUH Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17079-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, RIP, ELISA

Synonyms

3-MG-CoA hydratase, AU-binding protein/enoyl-CoA hydratase, AU-specific RNA-binding enoyl-CoA hydratase, EC:4.2.1.18, EC:4.2.1.56

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIPIHC

WB analysis of human brain using 17079-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 17079-1-AP (AUH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 17079-1-AP
WB result of AUH antibody (17079-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-AUH transfected HepG2 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 17079-1-AP
IP result of anti-AUH (IP:17079-1-AP, 4ug; Detection:17079-1-AP 1:300) with HeLa cells lysate 2800 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 17079-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17079-1-AP (AUH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 17079-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17079-1-AP (AUH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	human brain tissue, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17079-1-AP targets AUH in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11265 Product name: Recombinant human AUH protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-310 aa of BC020722 Sequence: MAAAVAAAPGALGSLHAGGARLVAACSAWLCPGLRLPGSLAGRRAGPAIWAQGWVPAAGGPAPKRGYSSEMKTEDELRVRHLEEENRGIVVLGINRAYGKNSLSKNLIKMLSKAVDALKSDKKVRTIIIRSEVPGIFCAANLPVPTIAAIDGLALGGGLELALACDIRVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGKEAKAVGLISHVLEQNQEGDAAYRKALDLAREFLPQGPVAMRVAKLAINQGMEVDLVTGLAIEEACYAQTIPTKDRLEGLLAFKEKRPPRYKGE Predict reactive species
Full Name	AU RNA binding protein/enoyl-Coenzyme A hydratase
Calculated Molecular Weight	310 aa, 33 kDa
Observed Molecular Weight	32 kDa
GenBank Accession Number	BC020722
Gene Symbol	AUH
Gene ID (NCBI)	549
RRID	AB_2061316
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13825
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for AUH antibody 17079-1-AP	Download protocol
IP protocol for AUH antibody 17079-1-AP	Download protocol
WB protocol for AUH antibody 17079-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
mouse	WB	Nat Commun Leucine catabolic enzyme AUH regulates BAT thermogenesis via PPARγ HMGylation and RNA-binding function in male mice Authors - Haizhou Jiang View Article
human	RIP	Int J Biol Macromol m5C modification of LINC00324 promotes angiogenesis in glioma through CBX3/VEGFR2 pathway Authors - Aini Pan View Article