Tested Applications
| Positive WB detected in | HeLa cells, mouse thymus tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
60098-1-Ig targets Aurora B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7532 Product name: Recombinant human AURKB protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-371 aa of BC013300 Sequence: QIQLFAGGRESSSALDPSSPPPFSLRMAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRRHFTIDDFEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKSQIEKEGVEHQLRREIEIQAHLHHPNILRLYNYFYDRRRIYLILEYAPRGELYKELQKSCTFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLGLKGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEKVDLWCIGVLCYELLVGNPPFESASHNETYRRIVKVDLKFPASVPMGAQDLISKLLRHNPSERLPLAQVSAHPWVRANSRRVLPPSALQSVA Predict reactive species |
| Full Name | aurora kinase B |
| Calculated Molecular Weight | 39.2 kDa |
| Observed Molecular Weight | 39-45 kDa |
| GenBank Accession Number | BC013300 |
| Gene Symbol | AURKB |
| Gene ID (NCBI) | 9212 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96GD4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Aurora B(AURKB) is also named as aurora kinase B, AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5 and belongs to the Aurora subfamily. Aurora kinase B is a chromosomal passenger serine/threonine protein kinase that regulates accurate chromosomal segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachments, and regulation of the mitotic checkpoint(PMID:18311747). Expression levels of AURKB are increased in the anaplastic thyroid carcinoma cell lines ARO, Cal 62, and KAT-4(PMID:15562011).
Publications
| Species | Application | Title |
|---|---|---|
Gastroenterology Overexpression of Rac GTPase Activating Protein 1 Contributes to Proliferation of Cancer Cells by Reducing Hippo Signaling to Promote Cytokinesis. | ||
Hum Mol Genet Downregulation of Survivin contributes to cell-cycle arrest during postnatal cardiac development in a severe spinal muscular atrophy mouse model. | ||
PLoS One Anti-pancreatic cancer deliverables from sea: first-hand evidence on the efficacy, molecular targets and mode of action for multifarious polyphenols from five different brown-algae. | ||
FEBS J YAP co-localizes with the mitotic spindle and midbody to safeguard mitotic division in lung-cancer cells |
