AZI2/NAP1 Polyclonal antibody

AZI2/NAP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15042-1-AP

Synonyms

5 azacytidine induced 2, AZ2, AZI2, AZI2/NAP1, Nak associated protein 1, NAP1, TILP



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells, HepG2 cells
Positive IHC detected inmouse testis tissue, human pancreas tissue, human stomach tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15042-1-AP targets AZI2/NAP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AZI2/NAP1 fusion protein Ag6892
Full Name 5-azacytidine induced 2
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC001139
Gene Symbol AZI2
Gene ID (NCBI) 64343
RRIDAB_2878103
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AZI2, also known as NAP1, is a TNF receptor (TNFR)-associated factor family member-associated NF-κB activator-binding kinase 1-binding protein that regulates the production of IFNs. It is an adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity.

Protocols

Product Specific Protocols
WB protocol for AZI2/NAP1 antibody 15042-1-APDownload protocol
IHC protocol for AZI2/NAP1 antibody 15042-1-APDownload protocol
IF protocol for AZI2/NAP1 antibody 15042-1-APDownload protocol
IP protocol for AZI2/NAP1 antibody 15042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Res

FIP200 suppresses immune checkpoint therapy responses in breast cancers by limiting AZI2/TBK1/IRF signaling independent of its canonical autophagy function.

Authors - Takako Okamoto
humanWB

PLoS Pathog

MAVS activates TBK1 and IKKε through TRAFs in NEMO dependent and independent manner.

Authors - Run Fang
humanWB

Front Immunol

Increased IKKϵ protein stability ensures efficient type I interferon responses in conditions of TBK1 deficiency

Authors - Julia Wegner
humanWB,IHC,IF

Autophagy

AZI2 mediates TBK1 activation at unresolved selective autophagy cargo receptor complexes with implications for CD8 T-cell infiltration in breast cancer

Authors - Syn Kok Yeo
humanWB

J Cell Biol

NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis

Authors - Swagatika Paul