Tested Applications
| Positive WB detected in | human liver tissue |
| Positive IHC detected in | human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15990-1-AP targets BAAT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8860 Product name: Recombinant human BAAT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-418 aa of BC009567 Sequence: MIQLTATPVSALVDEPVHIQATGLIPFQMVSFQASLEDENGDMFYSQAHYRANEFGEVDLNHASSLGGDYMGVHPMGLFWSLKPEKLLTRLLKRDVMNRPFQVQVKLYDLELIVNNKVASAPKASLTLERWYVAPGVTRIKVREGRLRGALFLPPGEGLFPGVIDLFGGLGGLLEFRASLLASRGFASLALAYHNYEDLPRKPEVTDLEYFEEAANFLLRHPKVFGSGVGVVSVCQGVQIGLSMAIYLKQVTATVLINGTNFPFGIPQVYHGQIHQPLPHSAQLISTNALGLLELYRTFETTQVGASQYLFPIEEAQGQFLFIVGEGDKTINSKAHAEQAIGQLKRHGKNNWTLLSYPGAGHLIEPPYSPLCCASTTHDLRLHWGGEVIPHAAAQEHAWKEIQRFLRKHLIPDVTSQL Predict reactive species |
| Full Name | bile acid Coenzyme A: amino acid N-acyltransferase (glycine N-choloyltransferase) |
| Calculated Molecular Weight | 418 aa, 46 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC009567 |
| Gene Symbol | BAAT |
| Gene ID (NCBI) | 570 |
| RRID | AB_2061361 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14032 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BAAT is bile acid-CoA:amino acid N-acyltransferase which involved in in bile acid metabolism. BAAT acts as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BAAT antibody 15990-1-AP | Download protocol |
| IHC protocol for BAAT antibody 15990-1-AP | Download protocol |
| WB protocol for BAAT antibody 15990-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
