Tested Applications
| Positive WB detected in | HEK-293 cells, Hela cells, SH-SY5Y cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
16321-1-AP targets BACE2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9404 Product name: Recombinant human BACE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 39-398 aa of BC014453 Sequence: TNRVVAPTPGPGTPAERHADGLALALEPALASPAGAANFLAMVDNLQGDSGRGYYLEMLIGTPPQKLQILVDTGSSNFAVAGTPHSYIDTYFDTERSSTYRSKGFDVTVKYTQGSWTGFVGEDLVTIPKGFNTSFLVNIATIFESENFFLPGIKWNGILGLAYATLAKPSSSLETFFDSLVTQANIPNVFSMQMCGAGLPVAGSGTNGGSLVLGGIEPSLYKGDIWYTPIKEEWYYQIEILKLEIGGQSLNLDCREYNADKAIVDSGTTLLRLPQKVFDAVVEAVARASLIPEFSDGFWTGSQLACWTNSETPWSYFPKISIYLRDENSSRSFRITILPQLYIQPMMGAGLNYECYRFGI Predict reactive species |
| Full Name | beta-site APP-cleaving enzyme 2 |
| Calculated Molecular Weight | 518 aa, 56 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC014453 |
| Gene Symbol | BACE2 |
| Gene ID (NCBI) | 25825 |
| RRID | AB_2061667 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5Z0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BACE2, also known as ASP-1 or memapsin 1, is the closest homologue of memapsin 2, with 50% residue identity. BACE2 is located on chromosome 21 in the Down's syndrome (DS) obligate region and may contribute to amyloid abnormalities in these individuals. BACE2 transcripts are expressed in the central nervous system and many peripheral tissues; however, its expression level in neurons is substantially lower than BACE1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BACE2 antibody 16321-1-AP | Download protocol |
| WB protocol for BACE2 antibody 16321-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Brain Behav Immun Elevated β-secretase 1 expression mediates CD4+ T cell dysfunction via PGE2 signalling in Alzheimer's disease. | ||
Int J Neurosci Activating transcription factor 6 reduces Aβ1-42 and restores memory in Alzheimer's disease model mice. |
