BAG1S-specific Antibody
Rabbit Polyclonal
Catalog number: 16150-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human, mouse; other species not tested. |
| Cited species | |
| Positive WB detected in | mouse brain tissue, Jurkat cells, mouse liver tissue |
| Positive IHC detected in | human testis |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16150-1-AP(BAG1S-specific Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human testis using 16150-1-AP(BAG1S-specific Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis using 16150-1-AP(BAG1S-specific Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Peptide | Full name | BCL2-associated athanogene |
|
Calculated molecular weight |
26kd |
Observed molecular
weight |
46kd,33kd |
|
GenBank accession number |
NM_004323 | Gene ID (NCBI) | 573 |
| Gene symbol | BAG1 | Synonyms | RAP46 |
Background
BAG1 have been identified that modulate gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody is specific to BAG1S. |
