BID Antibody
Rabbit Polyclonal
Catalog number: 10988-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | WB |
| Species specificity | human; other species not tested. |
| Cited species | human |
| Positive WB detected in | HepG2 cells, HEK-293 cells, A431 cells, A549 cells, HeLa cells, human brain tissue, Jurkat cells |
| Positive IHC detected in | human colon cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of BID antibody (10988-1-AP for Ip and Detection) with HeLa cell lysate.
HepG2 cells were subjected to SDS PAGE followed by western blot with 10988-1-AP(BID Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer using 10988-1-AP(BID Antibody) at Dilution 1:100 (under 10x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | BID fusion protein ag1445 | Full name | BH3 interacting domain death agonist |
|
Calculated molecular weight |
22kd |
Observed molecular
weight |
22 kDa |
|
GenBank accession number |
BC009197 | Gene ID (NCBI) | 637 |
| Gene symbol | BID | Synonyms | FP497; MGC15319; MGC42355 |
Background
BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606) |
