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  • Western Blot (WB) analysis of Jurkat cells using BIN3 Polyclonal antibody (20186-1-AP)

    WB analysis of Jurkat using 20186-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20186-1-AP (BIN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BIN3 Polyclonal antibody (20186-1-AP)

    WB analysis of HeLa using 20186-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20186-1-AP (BIN3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using BIN3 Polyclonal antibody (20186-1-AP)

    WB analysis of mouse testis using 20186-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20186-1-AP (BIN3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BIN3 Polyclonal antibody (20186-1-AP)

    WB analysis of HEK-293 using 20186-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20186-1-AP (BIN3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using BIN3 Polyclonal antibody (20186-1-AP)

    WB analysis of A375 using 20186-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 20186-1-AP (BIN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

BIN3 Polyclonal antibody

BIN3 Polyclonal Antibody for WB, ELISA
Cat No. 20186-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

BIN3, bridging integrator 3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inJurkat cells, A375 cells, HEK-293 cells, HeLa cells, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20186-1-AP targets BIN3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14091

Product name: Recombinant human BIN3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-199 aa of BC001223

Sequence: MSKSAVKISLDLLSNPLCEQDQDLLNMVTALDTAMKRMDAFNQEKVNQIQKTVIEPLKKFGSVFPSLNMAVKRREQALQDYRRLQAKVEKYEEKEKTGPVLAKLHQAREELRPVREDFEAKNRQLLEEMPRFYGSRLDYFQPSFESLIRAQVVYYSEMHKIFGDLSHQLDQPGHSDEQRERENEAKLSELRALSIVADD

Predict reactive species
Full Name bridging integrator 3
Calculated Molecular Weight 253 aa, 30 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC001223
Gene Symbol BIN3
Gene ID (NCBI) 55909
RRIDAB_10638782
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQY0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BIN3, also named as MEPCE and BCDIN3, belongs to the methyltransferase superfamily. It is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it.

Protocols

Product Specific Protocols
WB protocol for BIN3 antibody 20186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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