Bcl-xL Antibody
Rabbit Polyclonal
Catalog number: 10783-1-AP
Ask a question about this product click here
Loading price...
Applications
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | K-562 cells, human placenta tissue, Jurkat cells, Raji cells, RAW 264.7 cells, mouse placenta tissue |
| Positive IHC detected in | human colon cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of anti-BCL2L1 (10783-1-AP for IP and Detection) with K-562 cells lysate.
K-562 cells were subjected to SDS PAGE followed by western blot with 10783-1-AP(Bcl-xL Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer using 10783-1-AP(Bcl-xL Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Bcl-xL fusion protein ag1232 | Full name | BCL2-like 1 |
|
Calculated molecular weight |
26kd |
Observed molecular
weight |
30-35kd |
|
GenBank accession number |
BC019307 | Gene ID (NCBI) | 598 |
| Gene symbol | BCL2L1 | Synonyms | BCL-XL/S; BCL2L; BCLX; Bcl-X; DKFZp781P2092; bcl-xL; bcl-xS |
Background
BCL2L1 is a member of he BCL-2 protein family. BCL2L1 is expressed as two distinct isoforms, Bcl-xL and Bcl-xS, and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. This antibody can recognize Bcl-XL, Bcl-XS and Bcl-X(Beta). |
