Bcl-xL Antibody
Mouse Monoclonal
Catalog number: 66020-1-Ig
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human; other species not tested. |
| Cited species | |
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human testis |
| Positive IF detected in | Hela cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Jurkat cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig(Bcl-xL Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human testis using 66020-1-Ig(Bcl-xL Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis using 66020-1-Ig(Bcl-xL Antibody) at Dilution 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using BCL2L1 antibody 66020-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Mouse | Purification method | Protein A purification |
| Isotype | IgG2b | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Bcl-xL fusion protein ag18037 | Full name | BCL2-like 1 |
|
Calculated molecular weight |
26kd |
Observed molecular
weight |
30kD |
|
GenBank accession number |
BC019307 | Gene ID (NCBI) | 598 |
| Gene symbol | BCL2L1 | Synonyms | BCL-XL/S; BCL2L; BCLX; Bcl-X; DKFZp781P2092; bcl-xL; bcl-xS |
Background
BCL2L1 is a member of he BCL-2 protein family. BCL2L1 is expressed as two distinct isoforms, Bcl-xL and Bcl-xS, and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. |
