EDRF1 Polyclonal antibody

EDRF1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21883-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C10orf137, Erythroid differentiation-related factor 1, RP11 383C5.6

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 21883-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human brain using 21883-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of PC-3 using 21883-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 21883-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 21883-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse brain using 21883-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 21883-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 21883-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 21883-1-AP (EDRF1 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human cervical cancer using 21883-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21883-1-AP (EDRF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human cervical cancer using 21883-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21883-1-AP (EDRF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 21883-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 21883-1-AP (EDRF1 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 21883-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 21883-1-AP (EDRF1 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, human brain tissue, PC-3 cells, HEK-293 cells, mouse brain tissue, MCF-7 cells
Positive IHC detected in	human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21883-1-AP targets EDRF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16441 Product name: Recombinant human C10orf137 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 889-1238 aa of BC107479 Sequence: NFESIEDATNAALLLCNTGRLMRICAQAHCGAGDELKREFSPEEGLYYNKAIDYYLKALRSLGTRDIHPAVWDSVNWELSTTYFTMATLQQDYAPLSRKAQEQIEKEVSEAMMKSLKYCDVDSVSARQPLCQYRAATIHHRLASMYHSCLRNQVGDEHLRKQHRVLADLHYSKAAKLFQLLKDAPCELLRVQLERVAFAEFQMTSQNSNVGKLKTLSGALDIMVRTEHAFQLIQKELIEEFGQPKSGDAAAAADASPSLNREEVMKLLSIFESRLSFLLLQSIKLLSSTKKKTSNNIEDDTILKTNKHIYSQLLRATANKTATLLERINVIVHLLGQLAAGSAASSNAVQ Predict reactive species
Full Name	chromosome 10 open reading frame 137
Calculated Molecular Weight	1238 aa, 139 kDa
Observed Molecular Weight	140-160 kDa
GenBank Accession Number	BC107479
Gene Symbol	EDRF1
Gene ID (NCBI)	26098
RRID	AB_11042452
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q3B7T1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EDRF1, also named as C10orf137, is a transcription factor involved in erythroid differentiation. It regulates alpha- and gamma-globin gene synthesis by modulating DNA-binding activity of GATA-1 transcription factor. EDRF1 has four isoforms with MW 66 kDa, 89 kDa and 135-140 kDa.

Protocols

Product Specific Protocols
IHC protocol for EDRF1 antibody 21883-1-AP	Download protocol
WB protocol for EDRF1 antibody 21883-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	iScience Differential localization of dengue virus protease affects cell homeostasis and triggers to thrombocytopenia Authors - Lekha Gandhi View Article