Filter: AllWBIHCIF/ICC

WB analysis of A549 using 24975-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 24975-1-AP (C10orf68 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of COS-7 using 24975-1-AP
COS-7 cells were subjected to SDS PAGE followed by western blot with 24975-1-AP (C10orf68 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
IHC staining of human kidney using 24975-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24975-1-AP (C10orf68 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 24975-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24975-1-AP (C10orf68 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 24975-1-AP
Immunofluorescent analysis of HEK-293 cells using 24975-1-AP (C10orf68 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
IF Staining of HEK-293 using 24975-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using C10orf68 antibody (24975-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
IF Staining of HEK-293 using 24975-1-AP
Immunofluorescent analysis of HEK-293 cells using 24975-1-AP (C10orf68 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

C10orf68 Polyclonal antibody

Name: C10orf68 Polyclonal antibody
Brand: Proteintech
SKU: 24975-1-AP

C10orf68 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24975-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Protein BIOT2, Coiled-coil domain-containing protein 7, CCDC7, BIOT2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, COS-7 cells
Positive IHC detected in	human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24975-1-AP targets C10orf68 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, monkey samples.

Tested Reactivity	human, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21682 Product name: Recombinant human C10orf68 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-348 aa of BC125091 Sequence: MVPASASGEGLRELLLVQEGEVGADMSHAHNEVPNERLVVEHQESLSKTKLQVKKQETSTEQPLTTPDKEPNENLILRHQDSMSKSEMQVKEQRTLKGQRIITHDEESGENPMLKHQDSVSKIQVQLEIQETSEGEGRSIPDKNSMFVHQDSVSKLQMQEKKKITPGRERRNTRIVVPNENVISVHQDSKSKLQMQEKKQINSGVERHKTFPLEIKKKDISLEHLLPEEKVLLSRSESQTKKLQAKVTSRKKTDIESLRGALGRRLLNDEFKTQSKSFPGPDIEQLTDAFGRDILKDEFKTRSKSLPETDERLHSTTERGTINDAIKTQLKRKSYPETVLKHLKGVNG Predict reactive species
Full Name	chromosome 10 open reading frame 68
Calculated Molecular Weight	628 aa, 71 kDa
Observed Molecular Weight	65 kDa
GenBank Accession Number	BC125091
Gene Symbol	C10orf68
Gene ID (NCBI)	79741
RRID	AB_2879828
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96M83
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

chromosome 10 open reading frame 68 (C10orf68) is a 628 amino-acid protein, which may have three isoforms. The function of C10orf68 is still non-known and needs to be further studied.

Protocols

Product Specific Protocols
IF protocol for C10orf68 antibody 24975-1-AP	Download protocol
IHC protocol for C10orf68 antibody 24975-1-AP	Download protocol
WB protocol for C10orf68 antibody 24975-1-AP	Download protocol