C11orf30 Polyclonal antibody

C11orf30 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 26212-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

BRCA2-interacting transcriptional repressor EMSY, EMSY, GL002, Protein EMSY

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIF/ICC

WB analysis using 26212-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26212-1-AP (C11orf30 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 26212-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 26212-1-AP (C11orf30 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 26212-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using C11orf30 antibody (26212-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	HEK-293T cells, Jurkat cells, HeLa cells, MCF-7 cells
Positive IF/ICC detected in	MCF-7 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

26212-1-AP targets C11orf30 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag23974 Product name: Recombinant human C11orf30 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-171 aa of BC029375 Sequence: KKALATSMLTGEAGSLPSTHMVVAGMANSTPQQQKCRESCSSPSTVGSSLTTRKIDPPAVPATGQFMRIQNVGQKKAEESPAEIIIQAIPQYAIPCHSSSNVVVEPSGLLELNNFTSQQLDDEETAMEQDIDSSTEDGTEPSPSQSSAERS Predict reactive species
Full Name	chromosome 11 open reading frame 30
Observed Molecular Weight	140-150 kDa
GenBank Accession Number	BC029375
Gene Symbol	C11orf30
Gene ID (NCBI)	56946
RRID	AB_2880429
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q7Z589
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IF protocol for C11orf30 antibody 26212-1-AP	Download protocol
WB protocol for C11orf30 antibody 26212-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB,IHC	Cell EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion. Authors - Antonio Marzio KD Validated View Article
	WB,IHC	Eur J Med Res EMSY enhances glycolysis in ovarian cancer cells Authors - Shaosheng Wang View Article