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C11orf70 Polyclonal antibody

C11orf70 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20780-1-AP

Synonyms

CK070, C11orf70

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using C11orf70 Polyclonal antibody (20780-1-AP)

    WB analysis of mouse testis using 20780-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20780-1-AP (C11orf70 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using C11orf70 Polyclonal antibody (20780-1-AP)

    WB analysis of MCF-7 using 20780-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20780-1-AP (C11orf70 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using C11orf70 Polyclonal antibody (20780-1-AP)

    IHC staining of human testis using 20780-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20780-1-AP (C11orf70 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using C11orf70 Polyclonal antibody (20780-1-AP)

    IHC staining of human testis using 20780-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20780-1-AP (C11orf70 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C11orf70 Polyclonal antibody (20780-1-AP)

    IHC staining of human breast cancer using 20780-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20780-1-AP (C11orf70 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C11orf70 Polyclonal antibody (20780-1-AP)

    IHC staining of human breast cancer using 20780-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20780-1-AP (C11orf70 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C11orf70 Polyclonal antibody (20780-1-AP)

    IF Staining of HeLa using 20780-1-AP

    Immunofluorescent analysis of Hela cells, using C11orf70 antibody 20780-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse testis tissue, MCF-7 cells
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20780-1-AP is C11orf70 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C11orf70 fusion protein Ag14793
Full Name chromosome 11 open reading frame 70
Calculated Molecular Weight 267 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC006128
Gene Symbol C11orf70
Gene ID (NCBI) 85016
RRIDAB_10755147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C11orf70 antibody 20780-1-APDownload protocol
IHC protocol for C11orf70 antibody 20780-1-APDownload protocol
IF protocol for C11orf70 antibody 20780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols