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Tested Applications
| Positive WB detected in | PC-3 cells, mouse brain tissue |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25447-1-AP targets C15orf58 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20215 Product name: Recombinant human C15orf58 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-170 aa of BC127821 Sequence: MALPHDSNETSYLLPPNNEDWGGQTIPDFVYGQKDLTAEGIQWPRNAPGIPDALPQSPFDAALCSAWKQRVELGLFRYRLRELQTQILPGAVGFVAQLNVERGVQRRPPQTIKSVRQAFDPVQFNFNKIRPGEVLFRLHREPDLPGTLLQEDILVVINVSPLEWGHISEI Predict reactive species |
| Full Name | chromosome 15 open reading frame 58 |
| Calculated Molecular Weight | 385 aa, 42 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC127821 |
| Gene Symbol | C15orf58 |
| Gene ID (NCBI) | 390637 |
| RRID | AB_2880085 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZNW5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C15orf58 antibody 25447-1-AP | Download protocol |
| IF protocol for C15orf58 antibody 25447-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
